Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001694
UniProt IDQ99759
Primary gene name(s)MAP3K3
Synonym gene name(s)MAPKKK3, MEKK3
Protein nameMitogen-activated protein kinase kinase kinase 3
Protein functionComponent of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. {ECO:0000269|PubMed:12912994, ECO:0000269|PubMed:14661019, ECO:0000269|PubMed:14743216, ECO:0000269|PubMed:9006902}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99759
Gene Ontology
(Biological Process)
Complete annatation
blood vessel development [GO:0001568];
intracellular signal transduction [GO:0035556];
MAPK cascade [GO:0000165];
positive regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043123];
positive regulation of p38MAPK cascade [GO:1900745];
protein autophosphorylation [GO:0046777]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
MAP kinase kinase kinase activity [GO:0004709];
metal ion binding [GO:0046872];
protein kinase activity [GO:0004672]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829]
Protein-protein interaction110379
Phylogenetic treeQ99759
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.599923494975883.07741929062022e-062.14239554495279e-05
AZA vs. DISU0.4061977958644680.1130319565866960.665367610387901
AZA vs. IL7-0.397487948184520.03950164098032330.608180014807367
AZA vs. SAHA0.4637163725873510.05884336570056580.297253650995419
DISU vs. CD31.994278327304393.14291430436597e-073.41082003052647e-06
DISU vs. IL7-0.8130084694472740.001448850281034810.0350584558372387
DISU vs. SAHA0.05948723002657030.8488739175046910.959959391514459
DMSO vs. AZA0.008090809574941660.9615625971096711
DMSO vs. CD31.59412357787921.76838246701561e-061.2054607179e-05
DMSO vs. DISU-0.4006665200423010.1041553040666810.576642333682909
DMSO vs. IL7-0.397893153079290.02748832105769830.336079837749231
DMSO vs. SAHA0.4505014734379530.05796182518072330.27513154875549
HIV vs. Mock in Activation0.2538703157677950.7295525711970110.999983755607037
HIV vs. Mock in Latency0.03787827188017020.8188790937766620.999834320637052
IL7 vs. CD31.208972288630550.0002612341171849280.00133794830048536
SAHA vs. CD32.039692524625778.10625617742033e-076.74282057153687e-06
SAHA vs. IL70.8581689003869650.0005216523246586170.00710645037431532
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.159044 0.776947
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2C60 X-ray 1.2Å A=37-124.
2JRH NMR - A=42-126.
2O2V X-ray 1.8Å B=37-124.
2PPH NMR - A=42-126.
4Y5O X-ray 2.3Å B=1-124.
4YL6 X-ray 2.1Å B=1-22.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04722 Neurotrophin signaling pathway - Homo sapiens (human)
hsa04912 GnRH signaling pathway - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)