Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001693
UniProt IDQ9Y2U5
Primary gene name(s)MAP3K2
Synonym gene name(s)MAPKKK2, MEKK2
Protein nameMitogen-activated protein kinase kinase kinase 2
Protein functionComponent of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7, By similarity. Plays a role in caveolae kiss-and-run dynamics. {ECO:0000250, ECO:0000269|PubMed:10713157, ECO:0000269|PubMed:16001074}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:15075238}. Nucleus {ECO:0000269|PubMed:15075238}. Note=Upon EGF stimulation, translocates into the nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y2U5
Gene Ontology
(Biological Process)
Complete annatation
activation of JUN kinase activity [GO:0007257];
activation of MAPK activity [GO:0000187];
cellular response to mechanical stimulus [GO:0071260];
positive regulation of transcription, DNA-templated [GO:0045893];
protein phosphorylation [GO:0006468]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
MAP kinase kinase kinase activity [GO:0004709];
metal ion binding [GO:0046872];
protein kinase activity [GO:0004672];
protein kinase binding [GO:0019901];
protein serine/threonine kinase activity [GO:0004674];
receptor signaling protein serine/threonine kinase activity [GO:0004702]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654]
Protein-protein interaction115969
Phylogenetic treeQ9Y2U5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.759115856971860.02094012474475970.048398951104588
AZA vs. DISU0.5503522187027240.0301951722377390.411332385591532
AZA vs. IL70.09750308796610120.6116902697878350.999311006273513
AZA vs. SAHA-0.3075598251468440.2093274339964310.578336096052457
DISU vs. CD3-0.2219797351994140.5405628539138920.660541866074741
DISU vs. IL7-0.4612307113078430.0677287723012550.345169453158018
DISU vs. SAHA-0.8576168736765330.003439188732750620.0494108802586126
DMSO vs. AZA-0.1069668868698610.5228536896810351
DMSO vs. CD3-0.8773826354283770.006381294832470740.0169250363822485
DMSO vs. DISU-0.6591303935910810.007227455966460680.18155958255506
DMSO vs. IL70.2117170678277320.2388123035259670.744590584416536
DMSO vs. SAHA-0.2086089566803540.3783622133007270.725836311964274
HIV vs. Mock in Activation-0.1563427919439330.8021286446475710.999983755607037
HIV vs. Mock in Latency0.04262623436288750.7958708513013340.999834320637052
IL7 vs. CD3-0.6522161781491360.04284399842360250.0965591489591748
SAHA vs. CD3-1.093074515487140.002265298830612330.00720972616205444
SAHA vs. IL7-0.4101055547097660.09337487313295990.27632131193064
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0389935 0.83651
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.941 1.073 1.062 1.034 1.03
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB06616 Bosutinib approved unknown inhibitor

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2CU1 NMR - A=43-132.
2NPT X-ray 1.7Å B/D=26-123.
5EX0 X-ray 2.7Å D=256-265.
5HQ8 X-ray 1.7Å I/J=250-264.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 20174665

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04540 Gap junction - Homo sapiens (human)
hsa04912 GnRH signaling pathway - Homo sapiens (human)