Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001685
UniProt IDP49641
Primary gene name(s)MAN2A2
Synonym gene name(s)MANA2X
Protein nameAlpha-mannosidase 2x
Protein functionCatalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
Subcellular locationGolgi apparatus membrane {ECO:0000250};
Single-pass type II membrane protein {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P49641
Gene Ontology
(Biological Process)
Complete annatation
mannose metabolic process [GO:0006013];
N-glycan processing [GO:0006491];
protein deglycosylation [GO:0006517]
Gene Ontology
(Molecular Function)
Complete annatation
alpha-mannosidase activity [GO:0004559];
carbohydrate binding [GO:0030246];
hydrolase activity, hydrolyzing N-glycosyl compounds [GO:0016799];
mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity [GO:0004572];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
Golgi membrane [GO:0000139];
integral component of membrane [GO:0016021]
Protein-protein interaction110295
Phylogenetic treeP49641
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.08173937428794510.8035417950842140.866617669803883
AZA vs. DISU0.2352302943688830.3527546947096830.889061354591807
AZA vs. IL7-0.02895757085862340.8800946417915820.999311006273513
AZA vs. SAHA-0.2867230017444170.2402556199054850.611488775362705
DISU vs. CD30.1403287925836830.7019700596832220.791285793855811
DISU vs. IL7-0.2734572530302040.278212814894260.665759604048733
DISU vs. SAHA-0.5188106875410340.07534827464660230.347869841899206
DMSO vs. AZA0.1087847645239010.5153635074638561
DMSO vs. CD30.01515525861619450.9623191594346120.973629156271486
DMSO vs. DISU-0.12812791064240.5996994908661480.937580678966213
DMSO vs. IL7-0.1304365384893070.4675071989082130.877449081616199
DMSO vs. SAHA-0.4004058176056850.0899753742501520.35058457331059
HIV vs. Mock in Activation0.2127127234446640.7323902069430890.999983755607037
HIV vs. Mock in Latency0.1462353142424690.3747394226231790.999834320637052
IL7 vs. CD3-0.1052770011040370.7440376930217510.828706136622837
SAHA vs. CD3-0.3910006233862210.2737424617876020.386047061495336
SAHA vs. IL7-0.260125551426090.2857153356567740.533094809962892
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.6456 0.008909

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.196456 0.173777
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
202032_s_at 1.47 No downregulated in CD8+ cells
219999_at 1.32 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 processed by 12560567
Envelope transmembrane glycoprotein gp41 processed by 1736542
Envelope surface glycoprotein gp160; precursor processed by 2187500

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00510 N-Glycan biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)