Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001680
UniProt IDQ5TCQ9
Primary gene name(s)MAGI3
Synonym gene name(s)KIAA1634
Protein nameMembrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
Protein functionActs as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2. {ECO:0000269|PubMed:10748157}.
Subcellular locationCell membrane;
Peripheral membrane protein. Cell junction, tight junction. Nucleus {ECO:0000250}. Note=Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q5TCQ9
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
intracellular signal transduction [GO:0035556];
regulation of JNK cascade [GO:0046328];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
guanylate kinase activity [GO:0004385]
Gene Ontology
(Cellular Component)
Complete annatation
bicellular tight junction [GO:0005923];
membrane [GO:0016020];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction129277
Phylogenetic treeQ5TCQ9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.672649937463220.0002544232437434730.00109655908770421
AZA vs. DISU0.1585946543862310.7193332755243190.973374730783303
AZA vs. IL7-0.1842073277222840.6406078827391020.999311006273513
AZA vs. SAHA1.064144573368020.00312853948664260.0453894169602027
DISU vs. CD31.819638876234280.0002010975722520620.00104241568229972
DISU vs. IL7-0.350041246069390.4208506418532550.778255819412057
DISU vs. SAHA0.9056552370254190.02675867301064940.193605358110176
DMSO vs. AZA-0.07200684325707720.8437938984845581
DMSO vs. CD31.581441005641060.0001306861422522010.000570828278640014
DMSO vs. DISU-0.2345719056896130.5625769481418550.926462524427252
DMSO vs. IL7-0.1042766972499530.7681162668515340.952672575716091
DMSO vs. SAHA1.128502049641780.0003240407250248990.00819398954169505
HIV vs. Mock in Activation-0.2656585774194310.7433109070730910.999983755607037
HIV vs. Mock in Latency-0.3360648638940620.1508683956507470.999834320637052
IL7 vs. CD31.501073322905980.0009985165205579350.00423008418054237
SAHA vs. CD32.706100917941568.04197375359195e-101.35333686952887e-08
SAHA vs. IL71.244079207596460.0004073749223029170.00585392714851684
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.109171 0.60808
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.056 1.342 1.859 1.736 1.269
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3SOE X-ray 1.6Å A=601-691.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04015 Rap1 signaling pathway - Homo sapiens (human)