Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001676
UniProt IDQ13257
Primary gene name(s)MAD2L1
Synonym gene name(s)MAD2
Protein nameMitotic spindle assembly checkpoint protein MAD2A
Protein functionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. {ECO:0000269|PubMed:10700282, ECO:0000269|PubMed:11804586, ECO:0000269|PubMed:15024386}.
Subcellular locationNucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Note=Recruited by MAD1L1 to unattached kinetochores, Probable. Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13257
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
cell division [GO:0051301];
mitotic cell cycle checkpoint [GO:0007093];
mitotic sister chromatid segregation [GO:0000070];
mitotic spindle assembly checkpoint [GO:0007094];
negative regulation of apoptotic process [GO:0043066];
negative regulation of mitotic cell cycle [GO:0045930];
negative regulation of protein catabolic process [GO:0042177];
negative regulation of ubiquitin protein ligase activity [GO:1904667];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
positive regulation of mitotic cell cycle spindle assembly checkpoint [GO:0090267];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
sister chromatid cohesion [GO:0007062]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
protein homodimerization activity [GO:0042803]
Gene Ontology
(Cellular Component)
Complete annatation
condensed chromosome kinetochore [GO:0000777];
cytosol [GO:0005829];
kinetochore [GO:0000776];
mitotic spindle [GO:0072686];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
spindle pole [GO:0000922]
Protein-protein interaction110260
Phylogenetic treeQ13257
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5281837896674680.5931364316007520.69876891218866
AZA vs. DISU0.2644564276772030.4627452025759630.924841440034003
AZA vs. IL70.2401527460911250.3338935389477330.999311006273513
AZA vs. SAHA-0.06824895745561270.7815193125940260.941864331354349
DISU vs. CD3-0.2766053716691920.7737541297498210.843718876725174
DISU vs. IL7-0.03279720815286830.9273940279292910.986390893149553
DISU vs. SAHA-0.3326875253772560.3427114653691880.721049663547906
DMSO vs. AZA-0.1405801514321840.4799638287402361
DMSO vs. CD3-0.681213979580940.4871093273387110.596464440897153
DMSO vs. DISU-0.4074585816509340.2163170683394030.728091984191312
DMSO vs. IL70.3880672348002020.05196069057713870.447093270805003
DMSO vs. SAHA0.06460483906590990.7859465107864480.939630662728404
HIV vs. Mock in Activation-0.09997646976383690.9565960260569580.999983755607037
HIV vs. Mock in Latency0.07954407256537340.6365463155622160.999834320637052
IL7 vs. CD3-0.2782863031511820.7759675058122850.85162066514552
SAHA vs. CD3-0.6223164619149050.5224026989374880.629355557837393
SAHA vs. IL7-0.3128916431435280.2022001014001560.434926416738863
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.702212 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.065 0.844 0.738 0.655 0.82
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1DUJ NMR - A=11-195.
1GO4 X-ray 2.0Å A/B/C/D=1-205.
1KLQ NMR - A=11-205.
1S2H NMR - A=1-205.
2QYF X-ray 2.3Å A/C=1-205.
2V64 X-ray 2.9Å A/C/F=2-205# D/E/H=118-205.
2VFX X-ray 1.9Å A/B/C/D/E/F/G/H/I/J/K/L=1-205.
3GMH X-ray 3.9Å A/B/C/D/E/F/G/H/I/J/K/L=11-205.
5LCW EM 4.0Å Z=1-205.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 24488929

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)