Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001675
UniProt IDP20645
Primary gene name(s)M6PR
Synonym gene name(s)MPR46, MPRD
Protein nameCation-dependent mannose-6-phosphate receptor
Protein functionTransport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
Subcellular locationLysosome membrane;
Single-pass type I membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P20645
Gene Ontology
(Biological Process)
Complete annatation
endosome to lysosome transport [GO:0008333];
receptor-mediated endocytosis [GO:0006898];
secretion of lysosomal enzymes [GO:0033299]
Gene Ontology
(Molecular Function)
Complete annatation
mannose binding [GO:0005537];
mannose transmembrane transporter activity [GO:0015578];
transmembrane signaling receptor activity [GO:0004888]
Gene Ontology
(Cellular Component)
Complete annatation
endosome [GO:0005768];
integral component of plasma membrane [GO:0005887];
late endosome [GO:0005770];
lysosomal membrane [GO:0005765];
membrane [GO:0016020];
perinuclear region of cytoplasm [GO:0048471];
trans-Golgi network [GO:0005802];
trans-Golgi network membrane [GO:0032588];
transport vesicle [GO:0030133]
Protein-protein interaction110251
Phylogenetic treeP20645
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3444190021844510.2925614439062910.410184751865639
AZA vs. DISU-0.04522824670582950.8578248828730530.990362656437555
AZA vs. IL7-0.08446786330370560.6596282217092940.999311006273513
AZA vs. SAHA0.3391301966973590.1657876446526470.519343922588113
DISU vs. CD3-0.4026321396165140.2667755590773840.394919801011262
DISU vs. IL7-0.04821814396554740.8479474398676170.968761474498244
DISU vs. SAHA0.3858811192942380.1877453124165950.554262410074535
DMSO vs. AZA-0.01529952337252110.9270044897791911
DMSO vs. CD3-0.3727184300026630.2435565173157010.348516236071527
DMSO vs. DISU0.02761225579534710.9097297055226880.988283279918411
DMSO vs. IL7-0.06167935701357540.7309162970947080.946043030712005
DMSO vs. SAHA0.3482785182437440.1407458214488050.44968841955797
HIV vs. Mock in Activation0.0751366559478770.9038027194753890.999983755607037
HIV vs. Mock in Latency0.006909534340715750.9664999983006070.999834320637052
IL7 vs. CD3-0.4212021987155060.1897677529673930.309899170149243
SAHA vs. CD3-0.02955218449133910.9333919414083040.953641800476145
SAHA vs. IL70.4203986873501090.08539628268339140.260685209633342
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0831453 0.643525
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.043 0.982 0.996 1.023 1.001
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02755 1-3 Sugar Ring of Pentamannosyl 6-Phosphate experimental unknown unknown
DB02900 alpha-D-mannose 6-phosphate experimental unknown unknown
DB01272 Alglucosidase alfa approved yes binder

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1JUQ X-ray 2.2Å E/F/G/H=265-277.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)
hsa04145 Phagosome - Homo sapiens (human)