Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001656
UniProt IDQ8TDW0
Primary gene name(s)LRRC8C
Synonym gene name(s)AD158, FAD158
Protein nameVolume-regulated anion channel subunit LRRC8C
Protein functionNon-essential component of the volume-regulated anion channel, VRAC, also named VSOAC channel, an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member, LRRC8B, LRRC8C, LRRC8D or LRRC8E; channel characteristics depend on the precise subunit composition. {ECO:0000269|PubMed:24790029, ECO:0000269|PubMed:26824658}.
Subcellular locationCell membrane {ECO:0000269|PubMed:24790029};
Multi-pass membrane protein {ECO:0000305}. Endoplasmic reticulum membrane {ECO:0000305|PubMed:24790029}. Note=In the absence of LRRC8A, resides primarily in a cytoplasmic compartment, probably the endoplasmic reticulum. Requires LRRC8A for expression at the cell membrane. {ECO:0000269|PubMed:24790029}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8TDW0
Gene Ontology
(Biological Process)
Complete annatation
anion transmembrane transport [GO:0098656];
fat cell differentiation [GO:0045444]
Gene Ontology
(Molecular Function)
Complete annatation
volume-sensitive anion channel activity [GO:0005225]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
endoplasmic reticulum membrane [GO:0005789];
integral component of plasma membrane [GO:0005887];
membrane [GO:0016020];
plasma membrane [GO:0005886]
Protein-protein interaction123963
Phylogenetic treeQ8TDW0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9007228003861150.006454979637719020.0179766173502773
AZA vs. DISU0.07250224062403870.7742023530043090.976444104025318
AZA vs. IL7-0.005158584995533650.9813165680258080.999311006273513
AZA vs. SAHA-0.1504137071738370.5373844535879730.842226372778656
DISU vs. CD3-0.8413374087262980.02170319504711870.0550578865937845
DISU vs. IL7-0.08600615696826460.732503602800850.933273479734856
DISU vs. SAHA-0.2227657762944420.4442615459173440.788965483946508
DMSO vs. AZA-0.05442756278975050.7714127992135811
DMSO vs. CD3-0.9706912687333490.002671928493146010.00803445164655583
DMSO vs. DISU-0.1299849632254760.5939813398043730.936720258565498
DMSO vs. IL70.05702582570527150.7711544196406670.953428432386045
DMSO vs. SAHA-0.1029095513156720.6623644823621470.893649775454289
HIV vs. Mock in Activation-0.2595002367867490.6766910806607790.999983755607037
HIV vs. Mock in Latency-0.1491986659277260.3664728497646440.999834320637052
IL7 vs. CD3-0.8959556775819590.005894111991900490.0192548656380399
SAHA vs. CD3-1.07774084921430.002666082990436030.00829093492898698
SAHA vs. IL7-0.1504331389255260.5364601536709790.754628626020561
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.492648 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.175 1.374 1.155 1.62 0.981
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found