Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001643
UniProt IDO60711
Primary gene name(s)LPXN
Synonym gene name(s)LDLP
Protein nameLeupaxin
Protein functionTranscriptional coactivator for androgen receptor, AR and serum response factor, SRF. Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events. Suppresses the integrin-induced tyrosine phosphorylation of paxillin, PXN. May play a critical role as an adapter protein in the formation of the adhesion zone in osteoclasts. Negatively regulates B-cell antigen receptor, BCR signaling. {ECO:0000269|PubMed:17640867, ECO:0000269|PubMed:18451096, ECO:0000269|PubMed:18497331, ECO:0000269|PubMed:20543562}.
Subcellular locationCytoplasm. Cell junction, focal adhesion. Nucleus. Cytoplasm, perinuclear region {ECO:0000250}. Cell projection, podosome. Cell membrane. Note=Shuttles between the cytoplasm and nucleus. Recruited to the cell membrane following B-cell antigen receptor, BCR cross-linking in B-cells. Enhanced focal adhesion kinase activity, PTK2/FAK attenuates its nuclear accumulation and limits its ability to enhance serum response factor, SRF-dependent gene transcription. Targeting to focal adhesions is essential for its tyrosine phosphorylation in response to bombesin.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60711
Gene Ontology
(Biological Process)
Complete annatation
cell adhesion [GO:0007155];
negative regulation of B cell receptor signaling pathway [GO:0050859];
negative regulation of cell adhesion [GO:0007162];
protein complex assembly [GO:0006461];
regulation of cell adhesion mediated by integrin [GO:0033628];
regulation of transcription, DNA-templated [GO:0006355];
signal transduction [GO:0007165];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
transcription cofactor activity [GO:0003712];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cell projection [GO:0042995];
cytoplasm [GO:0005737];
focal adhesion [GO:0005925];
membrane [GO:0016020];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
podosome [GO:0002102]
Protein-protein interaction114801
Phylogenetic treeO60711
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3025701879829110.3575970416715430.479042095109189
AZA vs. DISU0.4360278981930010.08495070550940310.611239587039972
AZA vs. IL7-0.129176891467730.5005290633595670.999311006273513
AZA vs. SAHA-0.08544113505661650.7255761514107990.923428568543383
DISU vs. CD30.7250822745750560.04854386719954230.105530754855192
DISU vs. IL7-0.5748065443008490.02282099222889910.191871978723176
DISU vs. SAHA-0.5186989455893410.07561272913169840.348702399248417
DMSO vs. AZA0.05540674049514360.7399537617240981
DMSO vs. CD30.3461269257089550.2813498967883730.390037378772139
DMSO vs. DISU-0.3825533515881480.116937615851480.593749956496563
DMSO vs. IL7-0.1773585437687320.3226970295658710.799721223986312
DMSO vs. SAHA-0.1461336682531230.5344893775333110.830196579948867
HIV vs. Mock in Activation0.1171406657338140.8505225146139120.999983755607037
HIV vs. Mock in Latency0.06741885308409020.6819639330359270.999834320637052
IL7 vs. CD30.1792855373765320.5778588026199770.697994756442683
SAHA vs. CD30.1940027311806520.5842696021764050.684038448166441
SAHA vs. IL70.04159814964149280.8640721700998090.943820462042602
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.145227 0.325277
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.95 1.1 1.231 1.31 1.255
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1X3H NMR - A=260-326.
4XEF X-ray 2.5Å B/C/E/F=1-20.
4XEK X-ray 1.7Å C=86-104.
4XEV X-ray 2.0Å C/F=86-104.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found