Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001633
UniProt IDP49257
Primary gene name(s)LMAN1
Synonym gene name(s)ERGIC53, F5F8D
Protein nameProtein ERGIC-53
Protein functionMannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. {ECO:0000269|PubMed:12717434, ECO:0000269|PubMed:13130098}.
Subcellular locationEndoplasmic reticulum-Golgi intermediate compartment membrane;
Single-pass type I membrane protein. Golgi apparatus membrane;
Single-pass membrane protein. Endoplasmic reticulum membrane;
Single-pass type I membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P49257
Gene Ontology
(Biological Process)
Complete annatation
blood coagulation [GO:0007596];
COPII vesicle coating [GO:0048208];
endoplasmic reticulum organization [GO:0007029];
ER to Golgi vesicle-mediated transport [GO:0006888];
Golgi organization [GO:0007030];
positive regulation of organelle organization [GO:0010638];
protein folding [GO:0006457];
protein N-linked glycosylation via asparagine [GO:0018279];
protein transport [GO:0015031]
Gene Ontology
(Molecular Function)
Complete annatation
mannose binding [GO:0005537];
metal ion binding [GO:0046872];
unfolded protein binding [GO:0051082]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum-Golgi intermediate compartment [GO:0005793];
endoplasmic reticulum-Golgi intermediate compartment membrane [GO:0033116];
endoplasmic reticulum membrane [GO:0005789];
ER to Golgi transport vesicle membrane [GO:0012507];
extracellular exosome [GO:0070062];
Golgi membrane [GO:0000139];
host cell perinuclear region of cytoplasm [GO:0044220];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
sarcomere [GO:0030017]
Protein-protein interaction110185
Phylogenetic treeP49257
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.326444158073876.90279354839118e-050.000346911985199573
AZA vs. DISU0.1088748265348860.6740456852074320.96445964444266
AZA vs. IL70.3613885595287850.07395311815552330.761403927567078
AZA vs. SAHA0.2438606484585170.3180934877368190.688923605934606
DISU vs. CD3-1.230043347946440.0008176453781442160.0034787429946785
DISU vs. IL70.2441316867124070.396090613132080.760833270563048
DISU vs. SAHA0.1353879463531960.6476481814255360.891335222691649
DMSO vs. AZA-0.1091803146043050.5142883501297261
DMSO vs. CD3-1.447795237131279.182158921206e-065.33471747581829e-05
DMSO vs. DISU-0.2201280055914810.3702037919451830.85177548964527
DMSO vs. IL70.4779323428846040.007920722680306080.185638917408722
DMSO vs. SAHA0.3456392617122690.1434739860977570.455417916920067
HIV vs. Mock in Activation-0.1343561413173450.8301243572318460.999983755607037
HIV vs. Mock in Latency-0.01288216088948510.9376372455744140.999834320637052
IL7 vs. CD3-0.9555429103166290.003177656895438920.0114312225262412
SAHA vs. CD3-1.107963411094750.002097933938912220.00674399839151177
SAHA vs. IL7-0.1215989960296890.6183307706300970.80873909210222
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.3468 0.0494

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.164736 0.250263
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.027 0.983 1.031 1.078 0.887
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00025 Antihemophilic Factor (Recombinant) approved, investigational unknown chaperone

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3A4U X-ray 1.8Å A=31-285.
3LCP X-ray 2.4Å A/B=32-277.
3WHT X-ray 1.8Å A=31-269.
3WHU X-ray 2.6Å A=31-269.
3WNX X-ray 2.7Å A=31-269.
4GKX X-ray 2.7Å A/B/C/D/E/F=31-270.
4GKY X-ray 2.4Å A=31-270.
4YGB X-ray 1.6Å A/C=31-269.
4YGC X-ray 2.4Å A/C/E/G=31-269.
4YGD X-ray 2.5Å A/C/E/G=31-269.
4YGE X-ray 3.0Å A/C/E=31-269.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope transmembrane glycoprotein gp41 binds 12941144
Envelope surface glycoprotein gp160; precursor interacts with 22190034
Envelope transmembrane glycoprotein gp41 interacts with 22190034
Envelope surface glycoprotein gp120 binds 12487819

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)