Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001608
UniProt IDQ9P2J5
Primary gene name(s)LARS
Synonym gene name(s)KIAA1352
Protein nameLeucine--tRNA ligase, cytoplasmic
Protein functionCatalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid, AA is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs. {ECO:0000269|PubMed:19426743}.
Subcellular locationCytoplasm {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9P2J5
Gene Ontology
(Biological Process)
Complete annatation
leucyl-tRNA aminoacylation [GO:0006429];
tRNA aminoacylation for protein translation [GO:0006418]
Gene Ontology
(Molecular Function)
Complete annatation
aminoacyl-tRNA editing activity [GO:0002161];
ATP binding [GO:0005524];
leucine-tRNA ligase activity [GO:0004823]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleus [GO:0005634]
Protein-protein interaction119584
Phylogenetic treeQ9P2J5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3786652745562130.2478238530591540.35998468709595
AZA vs. DISU0.02049504055916450.9353031013480040.995042856396371
AZA vs. IL70.3940686455789240.04011070871780910.610858300940239
AZA vs. SAHA-0.693393660943680.004849140305101860.0609132841325879
DISU vs. CD3-0.3710954337250930.3068090061940420.438664418037465
DISU vs. IL70.3646822857199250.1474032693710140.500122141875431
DISU vs. SAHA-0.7124488105866060.01572592522748640.139067948575008
DMSO vs. AZA0.05571479200547510.7386361322496761
DMSO vs. CD3-0.3334034294612530.2971196886117040.407360221896966
DMSO vs. DISU0.03375928089024570.8897638881280150.987895678008431
DMSO vs. IL70.34545826443920.05425087627072660.458683095783064
DMSO vs. SAHA-0.7559532777128210.001485004946613190.0236580439222065
HIV vs. Mock in Activation-0.0325787736948910.958223527230730.999983755607037
HIV vs. Mock in Latency-0.02320864829741480.8878849787626570.999834320637052
IL7 vs. CD30.02321575595021250.9423399262155290.964414021445319
SAHA vs. CD3-1.096282302151370.002159224268043270.00691006789539437
SAHA vs. IL7-1.090681778604521.03319105682065e-050.000317843305928866
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.200986 0.164937
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.99 0.915 0.846 0.804 0.839
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00149 L-Leucine approved, nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2WFD X-ray 3.2Å A/B=260-509.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
matrix interacts with 22190034
Pr55(Gag) complexes with 23125841
HIV-1 virus replication enhanced by expression of human gene 18187620
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00970 Aminoacyl-tRNA biosynthesis - Homo sapiens (human)