Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001598
UniProt IDQ8IVT5
Primary gene name(s)KSR1
Synonym gene name(s)KSR
Protein nameKinase suppressor of Ras 1
Protein functionScaffolding protein that is part of a multiprotein signaling complex. Promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Does not have kinase activity by itself. {ECO:0000250|UniProtKB:Q61097}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:20679487}. Membrane {ECO:0000269|PubMed:20679487};
Peripheral membrane protein {ECO:0000269|PubMed:20679487}. Cell membrane {ECO:0000250|UniProtKB:Q61097};
Peripheral membrane protein {ECO:0000250|UniProtKB:Q61097}. Cell projection, ruffle membrane {ECO:0000250|UniProtKB:Q61097}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:20679487}. Note=In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. {ECO:0000305|PubMed:12007434}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8IVT5
Gene Ontology
(Biological Process)
Complete annatation
cAMP-mediated signaling [GO:0019933];
MAPK cascade [GO:0000165];
positive regulation of MAPK cascade [GO:0043410];
Ras protein signal transduction [GO:0007265]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
MAP-kinase scaffold activity [GO:0005078];
metal ion binding [GO:0046872];
protein C-terminus binding [GO:0008022];
protein kinase activity [GO:0004672]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
membrane [GO:0016020];
protein complex [GO:0043234]
Protein-protein interaction114371
Phylogenetic treeQ8IVT5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.34263602455370.01818167999335060.043060069274007
AZA vs. DISU0.3585780356094030.1599410346620060.74228791764011
AZA vs. IL7-0.01186645966460980.9545177754447180.999311006273513
AZA vs. SAHA0.3844888137732650.1177482887603370.436853976070225
DISU vs. CD3-0.9973890195511010.08453724547206510.1635203950014
DISU vs. IL7-0.380112996172840.134193269876830.478950468966487
DISU vs. SAHA0.0293012346924010.9205217220351490.981778779774182
DMSO vs. AZA-0.02150160844460380.9020305287414931
DMSO vs. CD3-1.373648551104670.01432095180815670.0337571331579786
DMSO vs. DISU-0.3812766518062230.121452029143210.602729645757499
DMSO vs. IL70.01688288218852370.92609586665150.984721904896444
DMSO vs. SAHA0.4005765374848910.09162691867434760.354442836586523
HIV vs. Mock in Activation0.1926867371544390.8694519438731490.999983755607037
HIV vs. Mock in Latency0.3268655939239840.05076638976831130.886944717661561
IL7 vs. CD3-1.350386937470270.01756068572851270.0472440440932485
SAHA vs. CD3-0.9812208285141720.07497443219925030.138277407079024
SAHA vs. IL70.3938780325291430.1084897585907860.301841015318847
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.589423 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04014 Ras signaling pathway - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)