Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001594
UniProt IDQ14974
Primary gene name(s)KPNB1
Synonym gene name(s)NTF97
Protein nameImportin subunit beta-1
Protein functionFunctions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals, NLS in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex, NPC is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding, BIB domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus. {ECO:0000269|PubMed:10228156, ECO:0000269|PubMed:11891849, ECO:0000269|PubMed:19386897, ECO:0000269|PubMed:24699649, ECO:0000269|PubMed:9687515}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:11891849}. Nucleus envelope {ECO:0000269|PubMed:11891849}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14974
Gene Ontology
(Biological Process)
Complete annatation
apoptotic DNA fragmentation [GO:0006309];
astral microtubule organization [GO:0030953];
establishment of mitotic spindle localization [GO:0040001];
establishment of protein localization [GO:0045184];
intracellular transport of virus [GO:0075733];
mitotic chromosome movement towards spindle pole [GO:0007079];
mitotic metaphase plate congression [GO:0007080];
mitotic spindle assembly [GO:0090307];
modulation by virus of host process [GO:0019054];
NLS-bearing protein import into nucleus [GO:0006607];
protein import into nucleus [GO:0006606];
protein import into nucleus, docking [GO:0000059];
protein import into nucleus, translocation [GO:0000060];
Ran protein signal transduction [GO:0031291];
ribosomal protein import into nucleus [GO:0006610]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
nuclear localization sequence binding [GO:0008139];
poly(A RNA binding [GO:0044822];
protein domain specific binding [GO:0019904];
protein transporter activity [GO:0008565];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endoplasmic reticulum tubular network [GO:0071782];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
nuclear envelope [GO:0005635];
nuclear membrane [GO:0031965];
nuclear periphery [GO:0034399];
nuclear pore [GO:0005643];
nucleoplasm [GO:0005654];
protein complex [GO:0043234]
Protein-protein interaction110035
Phylogenetic treeQ14974
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.847049244489184.37658880247938e-084.55866941471798e-07
AZA vs. DISU0.1894654445042810.4532943520678130.921380671935034
AZA vs. IL70.2942125050923020.1250513987525750.892098508590985
AZA vs. SAHA-0.1884230257037440.4390671429457880.786885996048604
DISU vs. CD3-1.669802995479196.88582630536949e-065.36141248590598e-05
DISU vs. IL70.09584633924434660.703209392718040.923741138299926
DISU vs. SAHA-0.3770334796025430.1954718454582940.56673858048447
DMSO vs. AZA-0.05937717554503440.7220392986924421
DMSO vs. CD3-1.917560772815916.3651419690558e-097.05598703192241e-08
DMSO vs. DISU-0.2507287032369590.3036929638658570.811498856723186
DMSO vs. IL70.3609426468463390.04428525332826920.423873138999148
DMSO vs. SAHA-0.1363496435902720.5622627055693120.845433839681773
HIV vs. Mock in Activation0.0172704890775380.9778444287685390.999983755607037
HIV vs. Mock in Latency0.01413992680387950.9314611396629250.999834320637052
IL7 vs. CD3-1.544664206092542.4784036865011e-062.20525258362639e-05
SAHA vs. CD3-2.060723088938781.88126383360299e-082.3566621755941e-07
SAHA vs. IL7-0.48631931775180.04584517010058640.173374333691982
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -0.996353872
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.366453 0.00570214
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.015 0.957 0.982 1.008 0.905
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1F59 X-ray 2.8Å A/B=1-442.
1IBR X-ray 2.3Å B/D=1-462.
1M5N X-ray 2.9Å S=1-485.
1O6O X-ray 2.8Å A/B/C=1-442.
1O6P X-ray 2.8Å A/B=1-442.
1QGK X-ray 2.5Å A=1-876.
1QGR X-ray 2.3Å A=1-876.
2P8Q X-ray 2.3Å A=1-876.
2Q5D X-ray 3.2Å A/B=1-876.
2QNA X-ray 2.8Å A=127-875.
3LWW X-ray 3.1Å A/C=1-876.
3W5K X-ray 2.6Å A=1-876.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
integrase interacts with 22417684
Rev binds 21401918
Gag-Pol complexes with 23125841
matrix imported by 8105392
integrase binds 9275210
integrase imported by 1631159
Rev inhibited by 9891055
HIV-1 virus replication enhanced by expression of human gene 18854154
Nef complexes with 23125841
Vpr interacts with 18445273
Rev interacts with 22174317
Envelope surface glycoprotein gp120 complexes with 23125841
Tat modulates 24742347
Tat binds 21321119
Tat imported by 9430704
Vpr regulates 9436978
Tat downregulates 18051367

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)