Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001593
UniProt IDP52292
Primary gene name(s)KPNA2
Synonym gene name(s)RCH1, SRP1
Protein nameImportin subunit alpha-1
Protein functionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex, NPC is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Subcellular locationCytoplasm {ECO:0000269|PubMed:7604027}. Nucleus {ECO:0000269|PubMed:7604027}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P52292
Gene Ontology
(Biological Process)
Complete annatation
DNA metabolic process [GO:0006259];
intracellular transport of virus [GO:0075733];
modulation by virus of host process [GO:0019054];
NLS-bearing protein import into nucleus [GO:0006607];
regulation of DNA recombination [GO:0000018]
Gene Ontology
(Molecular Function)
Complete annatation
histone deacetylase binding [GO:0042826];
nuclear localization sequence binding [GO:0008139];
poly(A RNA binding [GO:0044822];
protein transporter activity [GO:0008565]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
membrane [GO:0016020];
nuclear pore [GO:0005643];
nucleoplasm [GO:0005654]
Protein-protein interaction110036
Phylogenetic treeP52292
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.600231650667841.27009514017118e-133.48052253684727e-12
AZA vs. DISU0.2901815783957230.2523704631512460.833530315850544
AZA vs. IL70.2487325559375920.2509303997313060.999311006273513
AZA vs. SAHA-0.05872760272720030.8210191595048190.956222476866417
DISU vs. CD3-2.322445334039341.73381908830095e-093.27924578883921e-08
DISU vs. IL7-0.05028614903311490.8423461436018940.967173404182981
DISU vs. SAHA-0.3485054049033860.2376655569655240.616392636211911
DMSO vs. AZA-0.08519540261917990.666621580629051
DMSO vs. CD3-2.699084427803584.10782519111308e-151.26812267426472e-13
DMSO vs. DISU-0.3784316090019750.1220017783287440.603502148340218
DMSO vs. IL70.3417697769013440.05898006759951730.469550803169272
DMSO vs. SAHA0.01908658793014520.9361457938003610.9846317807625
HIV vs. Mock in Activation0.006610588303752430.9917326299272770.999983755607037
HIV vs. Mock in Latency-0.07613758645555310.7157930006091320.999834320637052
IL7 vs. CD3-2.342631101073574.8464565693962e-121.27265923119353e-10
SAHA vs. CD3-2.685472884280618.96061003174964e-132.71157416021018e-11
SAHA vs. IL7-0.3113888060353710.2035463748051110.43682489376598
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2.1 0.004425004 1.3 0.151060893 1.4 0.180620898
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.506776 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.08 1.123 1.654 1.983 0.988
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1EFX X-ray 3.0Å C=204-212.
1QGK X-ray 2.5Å B=11-54.
1QGR X-ray 2.3Å B=28-54.
3FEX X-ray 3.5Å C=70-529.
3FEY X-ray 2.2Å C=70-529.
3WPT X-ray 2.6Å A/B=75-497.
4E4V X-ray 2.5Å A/B=70-529.
4WV6 X-ray 1.7Å A=60-529.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05164 Influenza A - Homo sapiens (human)