Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001586
UniProt IDO95198
Primary gene name(s)KLHL2
Synonym gene name(s)unknown
Protein nameKelch-like protein 2
Protein functionComponent of a cullin-RING-based BCR, BTB-CUL3-RBX1 E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, leading most often to their proteasomal degradation, By similarity. Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4. Plays a role in the reorganization of the actin cytoskeleton. Promotes growth of cell projections in oligodendrocyte precursors. {ECO:0000250, ECO:0000269|PubMed:15715669, ECO:0000269|PubMed:23838290}.
Subcellular locationCytoplasm, cytoskeleton. Cell projection, ruffle. Cell projection {ECO:0000250}. Cell projection, lamellipodium {ECO:0000250}. Cytoplasm, cytosol. Note=A proportion colocalizes with the actin cytoskeleton. When over-expressed, colocalizes with NPTXR in perinuclear aggresomes, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95198
Gene Ontology
(Biological Process)
Complete annatation
protein ubiquitination [GO:0016567]
Gene Ontology
(Molecular Function)
Complete annatation
actin binding [GO:0003779]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
Cul3-RING ubiquitin ligase complex [GO:0031463];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
lamellipodium [GO:0030027];
ruffle [GO:0001726]
Protein-protein interaction116431
Phylogenetic treeO95198
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.844473280056855.73558693872656e-085.81350143513696e-07
AZA vs. DISU0.3323903410812360.1917357497817730.778826378930328
AZA vs. IL70.289341500737160.135509852821120.898662602392564
AZA vs. SAHA0.01114247463847530.9637515846022860.990968034535802
DISU vs. CD3-1.52532162481465.04027588184019e-050.000311381633782209
DISU vs. IL7-0.05227263268493230.8362004614924130.965836707606723
DISU vs. SAHA-0.3194657228134870.2760122431698630.655982498903153
DMSO vs. AZA-0.059011402901180.7278630881888931
DMSO vs. CD3-1.911685817879229.16487175040004e-099.8620566371269e-08
DMSO vs. DISU-0.3924173287370230.1101552025080480.585118596768977
DMSO vs. IL70.3551536575889250.0502932388747180.441261209409319
DMSO vs. SAHA0.06275702990743070.791109995571180.941176092773387
HIV vs. Mock in Activation0.2518824062355780.6966830910450990.999983755607037
HIV vs. Mock in Latency0.29338750473070.07768282633563860.999834320637052
IL7 vs. CD3-1.548344736336272.9823743511459e-062.59845830027874e-05
SAHA vs. CD3-1.857506941188673.90979631004207e-073.50509401501094e-06
SAHA vs. IL7-0.2808599636188210.2512177687085910.494561400746154
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.836454 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2XN4 X-ray 1.9Å A/B=294-591.
4CHB X-ray 1.5Å A/B=294-591.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found