Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001584
UniProt IDQ9H0H3
Primary gene name(s)KLHL25
Synonym gene name(s)ENC2
Protein nameKelch-like protein 25
Protein functionSubstrate-specific adapter of a BCR, BTB-CUL3-RBX1 E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25 ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1, 4E-BP1: ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1, 4E-BP1 probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25 complex does not target EIF4EBP1, 4E-BP1 when it is hyperphosphorylated or associated with eIF4E. {ECO:0000269|PubMed:22578813}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H0H3
Gene Ontology
(Biological Process)
Complete annatation
protein ubiquitination [GO:0016567];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
regulation of translational initiation [GO:0006446]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
Cul3-RING ubiquitin ligase complex [GO:0031463];
cytoplasm [GO:0005737]
Protein-protein interaction122162
Phylogenetic treeQ9H0H3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.516678924321270.1183721866841440.201480022326755
AZA vs. DISU0.1027204957553390.724073711063990.974208899704183
AZA vs. IL7-0.2204196174033120.3331330639379110.999311006273513
AZA vs. SAHA0.01065884544047580.9675405348030930.991228805411965
DISU vs. CD30.6081978114136840.0966963456084580.181564613067002
DISU vs. IL7-0.3332522983493930.1983176659648960.572807329052325
DISU vs. SAHA-0.08934621025727510.7624996292372250.933520057858182
DMSO vs. AZA0.05889357743657670.781116895124421
DMSO vs. CD30.5652349712206220.07993324103266390.142188484609409
DMSO vs. DISU-0.04552127987273410.8561804582780690.983807945138555
DMSO vs. IL7-0.2718076374569760.1394881221134880.638506914864208
DMSO vs. SAHA-0.05302603296490920.8240888483345020.95214987988725
HIV vs. Mock in Activation0.1670729168276410.790165510044590.999983755607037
HIV vs. Mock in Latency0.03613296429709460.8337768623498580.999834320637052
IL7 vs. CD30.3005702166466790.3532643437989210.490427745234376
SAHA vs. CD30.5046136182914750.15710978428210.249672513880761
SAHA vs. IL70.2299173004159280.3519542967959150.601561334890669
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.103851 0.576634
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found