Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001573
UniProt IDQ13118
Primary gene name(s)KLF10
Synonym gene name(s)TIEG, TIEG1
Protein nameKrueppel-like factor 10
Protein functionTranscriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis, By similarity. May play a role in the cell cycle regulation. {ECO:0000250|UniProtKB:O89091, ECO:0000269|PubMed:8584037}.
Subcellular locationNucleus {ECO:0000250|UniProtKB:O89091}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13118
Gene Ontology
(Biological Process)
Complete annatation
bone mineralization [GO:0030282];
cell-cell signaling [GO:0007267];
cell proliferation [GO:0008283];
cellular response to peptide [GO:1901653];
cellular response to starvation [GO:0009267];
circadian rhythm [GO:0007623];
negative regulation of cell proliferation [GO:0008285];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
positive regulation of osteoclast differentiation [GO:0045672];
regulation of circadian rhythm [GO:0042752];
skeletal system development [GO:0001501];
somatic stem cell population maintenance [GO:0035019];
transcription, DNA-templated [GO:0006351];
transforming growth factor beta receptor signaling pathway [GO:0007179]
Gene Ontology
(Molecular Function)
Complete annatation
core promoter sequence-specific DNA binding [GO:0001046];
metal ion binding [GO:0046872];
transcription factor activity, sequence-specific DNA binding [GO:0003700]
Gene Ontology
(Cellular Component)
Complete annatation
nucleus [GO:0005634]
Protein-protein interaction112927
Phylogenetic treeQ13118
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
      Negatively associated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD33.450068571233139.7352474304202e-089.41177994042933e-07
AZA vs. DISU2.271494312088030.01647895460082180.310117055746302
AZA vs. IL70.4455584723244540.09507514545443610.824387308020595
AZA vs. SAHA1.170317199572270.0001179307797211090.0042732898401827
DISU vs. CD3-1.193643033387950.3682759221922750.502297434955335
DISU vs. IL7-1.833167600276270.05088048401622360.297757825428865
DISU vs. SAHA-1.099109908649150.2855146479007590.667602387147823
DMSO vs. AZA-0.01409150338721790.9590530015286841
DMSO vs. CD3-3.484309392015381.01399089125209e-078.92276447237895e-07
DMSO vs. DISU-2.289796319414560.0160167901219280.264663406948964
DMSO vs. IL70.4683268020722410.1057170570788320.588269704946874
DMSO vs. SAHA1.179088973172470.0002739717216104780.00720598222755935
HIV vs. Mock in Activation-0.2226558807138610.8556383581184080.999983755607037
HIV vs. Mock in Latency-0.1897414563633220.2659351621738960.999834320637052
IL7 vs. CD3-2.99441099123954.30814536989121e-063.61764207021561e-05
SAHA vs. CD3-2.30627942461070.0002244387269308980.000974723808313726
SAHA vs. IL70.7205368807576750.02491609936050940.115938998459433
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.679617 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2EPA NMR - A=359-423.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat upregulates 25613138
Envelope surface glycoprotein gp120 upregulates 24162774

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found