Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001567
UniProt IDQ12840
Primary gene name(s)KIF5A
Synonym gene name(s)NKHC1
Protein nameKinesin heavy chain isoform 5A
Protein functionMicrotubule-dependent motor required for slow axonal transport of neurofilament proteins, NFH, NFM and NFL. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons. {ECO:0000250|UniProtKB:P33175}.
Subcellular locationCytoplasm, perinuclear region {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Concentrated in the cell body of the neurons, particularly in the perinuclear region. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q12840
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
axon guidance [GO:0007411];
chemical synaptic transmission [GO:0007268];
cytoskeleton-dependent intracellular transport [GO:0030705];
microtubule-based movement [GO:0007018];
protein localization [GO:0008104];
retrograde vesicle-mediated transport, Golgi to ER [GO:0006890];
vesicle-mediated transport [GO:0016192]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
ATP-dependent microtubule motor activity, plus-end-directed [GO:0008574];
motor activity [GO:0003774]
Gene Ontology
(Cellular Component)
Complete annatation
ciliary rootlet [GO:0035253];
cytosol [GO:0005829];
kinesin complex [GO:0005871];
membrane [GO:0016020];
microtubule [GO:0005874];
neuronal cell body [GO:0043025];
neuron projection [GO:0043005];
perinuclear region of cytoplasm [GO:0048471]
Protein-protein interaction109999
Phylogenetic treeQ12840
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.701256088314250.001503827941945810.00519140969789448
AZA vs. DISU0.2180133297338940.5813347705541010.955158276076142
AZA vs. IL7-0.2099482018935640.5850993115708550.999311006273513
AZA vs. SAHA0.7890112049943030.03960837924682990.235154276788781
DISU vs. CD31.906789184117730.0002967249949373810.00146123246445151
DISU vs. IL7-0.4388916162718350.2306483233365820.614616495243197
DISU vs. SAHA0.5754693617702540.1108050770913560.428080829747202
DMSO vs. AZA0.1005725364095280.7895294716444431
DMSO vs. CD31.797073292624820.0005548862224964420.00204828370664174
DMSO vs. DISU-0.1173611022135740.7457977629203640.969482258605607
DMSO vs. IL7-0.3040822621327610.3825876880180390.835526886123764
DMSO vs. SAHA0.6831262116065320.04868167343230630.248705593321454
HIV vs. Mock in Activation0.898587965629120.2886835105561950.999983755607037
HIV vs. Mock in Latency0.6549544434688720.0001991515398637180.0190000652652258
IL7 vs. CD31.492688613565270.003367267797104170.0120037185123172
SAHA vs. CD32.468728968738342.24595450870613e-061.67833130409612e-05
SAHA vs. IL70.9989189647422690.004884853109874520.03766459119092
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
NOTEST -0.211845 1
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4UXT EM 7.4Å C=1-340.
4UXY EM 6.5Å C=1-340.
4UY0 EM 7.7Å C=1-340.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
hsa04728 Dopaminergic synapse - Homo sapiens (human)