Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001565
UniProt IDO95239
Primary gene name(s)KIF4A
Synonym gene name(s)KIF4
Protein nameChromosome-associated kinesin KIF4A
Protein functionMotor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. {ECO:0000269|PubMed:15297875, ECO:0000269|PubMed:15625105}.
Subcellular locationNucleus matrix. Cytoplasm, cytoskeleton, spindle. Midbody. Chromosome. Note=Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95239
Gene Ontology
(Biological Process)
Complete annatation
anterograde axonal transport [GO:0008089];
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
microtubule-based movement [GO:0007018];
mitotic cytokinesis [GO:0000281];
mitotic spindle midzone assembly [GO:0051256];
organelle organization [GO:0006996];
retrograde vesicle-mediated transport, Golgi to ER [GO:0006890]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
ATP-dependent microtubule motor activity, plus-end-directed [GO:0008574];
DNA binding [GO:0003677];
microtubule motor activity [GO:0003777]
Gene Ontology
(Cellular Component)
Complete annatation
axon cytoplasm [GO:1904115];
chromosome [GO:0005694];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
kinesin complex [GO:0005871];
membrane [GO:0016020];
midbody [GO:0030496];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
spindle microtubule [GO:0005876]
Protein-protein interaction117288
Phylogenetic treeO95239
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1752976875622450.8871947108596680.926454033529658
AZA vs. DISU-0.3292910359256490.6591934775419270.960561603296512
AZA vs. IL7-0.03588674967823130.9627477454485880.999311006273513
AZA vs. SAHA-0.1919640035737620.7780200738521420.941353350035522
DISU vs. CD3-0.1660241314555250.8917713046992030.928488004976935
DISU vs. IL70.2831191572690710.6775122108862190.912716338636114
DISU vs. SAHA0.1384567230385650.8060556456645820.949661087852635
DMSO vs. AZA0.1273751037546620.8691270132237751
DMSO vs. CD30.2864140746248070.8149626093333910.869751467101696
DMSO vs. DISU0.4525448766912920.5079631283500450.91095124911794
DMSO vs. IL7-0.1549850546156940.8270600346331010.963051812112721
DMSO vs. SAHA-0.3237958708906490.5911656775675250.860404723548917
HIV vs. Mock in Activation0.4784502537510140.8274888620174490.999983755607037
HIV vs. Mock in Latency0.2751761453356250.5836471319914820.999834320637052
IL7 vs. CD30.1462834008066250.9050627406867330.939532416692226
SAHA vs. CD3-0.04217915873477250.9729072536867710.981569813750586
SAHA vs. IL7-0.1583527806824570.7921844594892720.909335100696107
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.337219 0.0143065
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1 1.174 1.506 1.598 1.232
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
218355_at 1.73 No upregulated in CD4+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) binds 10559369
Pr55(Gag) relocalized by 10559369
matrix interacts with 24237699
Pr55(Gag) co-localizes with 18684836
matrix binds 10559369
matrix relocalized by 10559369
Pr55(Gag) interacts with 24237699

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found