Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001561
UniProt IDQ99661
Primary gene name(s)KIF2C
Synonym gene name(s)KNSL6
Protein nameKinesin-like protein KIF2C
Protein functionIn complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells, PubMed:21820309. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis, PubMed:19060894. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment, PubMed:23891108. {ECO:0000269|PubMed:19060894, ECO:0000269|PubMed:21820309, ECO:0000269|PubMed:23891108}.
Subcellular locationCytoplasm, cytoskeleton {ECO:0000269|PubMed:19632184, ECO:0000269|PubMed:21820309, ECO:0000269|PubMed:23891108}. Nucleus {ECO:0000250|UniProtKB:P70096}. Chromosome, centromere {ECO:0000269|PubMed:14960279, ECO:0000269|PubMed:17485487}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:14960279, ECO:0000269|PubMed:17485487, ECO:0000269|PubMed:23891108}. Note=Associates with the microtubule network at the growing distal tip, the plus-end of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1, By similarity. Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. {ECO:0000250|UniProtKB:P70096, ECO:0000269|PubMed:17485487, ECO:0000269|PubMed:21820309}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99661
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
attachment of mitotic spindle microtubules to kinetochore [GO:0051315];
cell division [GO:0051301];
cell proliferation [GO:0008283];
establishment or maintenance of microtubule cytoskeleton polarity [GO:0030951];
metaphase plate congression [GO:0051310];
microtubule-based movement [GO:0007018];
microtubule depolymerization [GO:0007019];
mitotic metaphase plate congression [GO:0007080];
mitotic nuclear division [GO:0007067];
regulation of chromosome segregation [GO:0051983];
retrograde vesicle-mediated transport, Golgi to ER [GO:0006890];
sister chromatid cohesion [GO:0007062]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activity [GO:0016887];
ATP binding [GO:0005524];
centromeric DNA binding [GO:0019237];
microtubule motor activity [GO:0003777];
microtubule plus-end binding [GO:0051010]
Gene Ontology
(Cellular Component)
Complete annatation
chromosome, centromeric region [GO:0000775];
condensed chromosome kinetochore [GO:0000777];
cytosol [GO:0005829];
kinesin complex [GO:0005871];
membrane [GO:0016020];
microtubule cytoskeleton [GO:0015630];
microtubule plus-end [GO:0035371];
nucleus [GO:0005634]
Protein-protein interaction116195
Phylogenetic treeQ99661
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5965001181471450.6018204868811360.706270994181459
AZA vs. DISU-0.1805964272168990.7396014647228040.974754980471983
AZA vs. IL70.09293612259903810.8855783654001150.999311006273513
AZA vs. SAHA-0.7009211017469750.268491951576130.642114497550149
DISU vs. CD3-0.7861517566195960.497538037145760.623795090404657
DISU vs. IL70.2626062170399770.5750229341624120.864082010815228
DISU vs. SAHA-0.5181813459025290.2377527018594220.616406497457998
DMSO vs. AZA0.03626380659610620.9565965087762841
DMSO vs. CD3-0.5743982033305280.6139402624008920.708031084212619
DMSO vs. DISU0.2132414488648930.666327548690660.952992468973363
DMSO vs. IL70.06458348884362620.9159053017669470.98251422643825
DMSO vs. SAHA-0.7430546689793720.2120401912775030.554512879185016
HIV vs. Mock in Activation0.1071944655908770.9586523500503170.999983755607037
HIV vs. Mock in Latency0.4704727965033250.3201794146441910.999834320637052
IL7 vs. CD3-0.4961102629237920.6624514021508030.768086921893773
SAHA vs. CD3-1.323600486104070.2499143318802290.359165456936396
SAHA vs. IL7-0.7966889122470460.1664657295364150.388858578495572
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.8 0.00214775 1.3 0.07965598 1.3 0.320505067
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.43113 0.00132191
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.226 1.187 1.324 1.22 0.955
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
209408_at 1.5 Yes upregulated in CD4+ cells
209408_at 2.4 Yes upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB04395 Phosphoaminophosphonic Acid-Adenylate Ester experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2HEH X-ray 2.1Å A=225-593.
4UBF X-ray 3.0Å A/B/C/D=225-593# P=709-720.
4Y05 X-ray 2.5Å A=216-599.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat downregulates 16751065

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found