Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001559
UniProt IDQ02241
Primary gene name(s)KIF23
Synonym gene name(s)KNSL5, MKLP1
Protein nameKinesin-like protein KIF23
Protein functionComponent of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. {ECO:0000269|PubMed:16103226, ECO:0000269|PubMed:16236794, ECO:0000269|PubMed:22522702}.
Subcellular locationNucleus. Cytoplasm, cytoskeleton, spindle. Midbody. Note=Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q02241
Gene Ontology
(Biological Process)
Complete annatation
actomyosin contractile ring assembly [GO:0000915];
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
cytokinesis [GO:0000910];
microtubule-based movement [GO:0007018];
mitotic cytokinesis [GO:0000281];
mitotic spindle elongation [GO:0000022];
mitotic spindle midzone assembly [GO:0051256];
plus-end-directed vesicle transport along microtubule [GO:0072383];
positive regulation of cytokinesis [GO:0032467];
retrograde vesicle-mediated transport, Golgi to ER [GO:0006890]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activity [GO:0016887];
ATP binding [GO:0005524];
microtubule binding [GO:0008017];
microtubule motor activity [GO:0003777]
Gene Ontology
(Cellular Component)
Complete annatation
centralspindlin complex [GO:0097149];
centrosome [GO:0005813];
cytosol [GO:0005829];
focal adhesion [GO:0005925];
intercellular bridge [GO:0045171];
kinesin complex [GO:0005871];
microtubule [GO:0005874];
midbody [GO:0030496];
mitotic spindle [GO:0072686];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
spindle [GO:0005819]
Protein-protein interaction114873
Phylogenetic treeQ02241
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.2889266448856130.7924303639399350.858319112130989
AZA vs. DISU-0.4294696834302830.5643534200119880.951925020821155
AZA vs. IL70.05713135470923210.942001694305890.999311006273513
AZA vs. SAHA0.06693606164523350.9245625846012170.982479497044848
DISU vs. CD3-0.7305724520281870.4906115906056450.617370324466942
DISU vs. IL70.4770888206531690.4672593798861330.805738684379045
DISU vs. SAHA0.4952063423463750.3424809335341330.720884099063257
DMSO vs. AZA0.0340550480750170.9661709063365451
DMSO vs. CD3-0.2691066773239650.8024970100680520.860164884857717
DMSO vs. DISU0.4600845670148680.4964366602730090.907374223203668
DMSO vs. IL70.03100724322906710.9658987984244130.993414945980946
DMSO vs. SAHA0.02468915437529840.9683463685465280.993123383488583
HIV vs. Mock in Activation0.2818893204162230.8861713523255940.999983755607037
HIV vs. Mock in Latency0.1386387994255940.7531976097217840.999834320637052
IL7 vs. CD3-0.2223549017859430.836136911760940.894407186925886
SAHA vs. CD3-0.2498884075217820.820374191505380.872664928332556
SAHA vs. IL70.005479580293978770.9926746255418980.996648907214078
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
3.1 0.000419637 1.7 0.027444465 1.7 0.076573685
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.56854 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.021 5.57E-05

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.007 0.974 1.545 1.642 0.812
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3VHX X-ray 2.8Å B/D/F/H=794-911.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05206 MicroRNAs in cancer - Homo sapiens (human)