Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001544
UniProt IDQ2YD98
Primary gene name(s)UVSSA
Synonym gene name(s)KIAA1530
Protein nameUV-stimulated scaffold protein A
Protein functionFactor involved in transcription-coupled nucleotide excision repair, TC-NER in response to UV damage. TC-NER allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Acts by promoting stabilization of ERCC6 by recruiting deubiquitinating enzyme USP7 to TC-NER complexes, preventing UV-induced degradation of ERCC6 by the proteasome. Interacts with the elongating form of RNA polymerase II, RNA pol IIo and facilitates its ubiquitination at UV damage sites, leading to promote RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery. Not involved in processing oxidative damage. {ECO:0000269|PubMed:22466610, ECO:0000269|PubMed:22466611, ECO:0000269|PubMed:22466612}.
Subcellular locationChromosome {ECO:0000269|PubMed:22466611, ECO:0000269|PubMed:22466612}. Note=Accumulates at UV DNA damage sites.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q2YD98
Gene Ontology
(Biological Process)
Complete annatation
protein ubiquitination [GO:0016567];
response to UV [GO:0009411];
transcription-coupled nucleotide-excision repair [GO:0006283]
Gene Ontology
(Molecular Function)
Complete annatation
RNA polymerase II core binding [GO:0000993]
Gene Ontology
(Cellular Component)
Complete annatation
chromosome [GO:0005694];
nucleoplasm [GO:0005654]
Protein-protein interaction121689
Phylogenetic treeQ2YD98
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.8317694422352640.0119055182169970.0302699005679115
AZA vs. DISU-0.3421635751747110.1790865183513970.764471596372382
AZA vs. IL7-0.1371470616672210.4799142721025480.999311006273513
AZA vs. SAHA0.01111061699922390.9638770139560480.990993714666692
DISU vs. CD30.4770080032880310.1901556930558840.306862960831217
DISU vs. IL70.1954032082984150.4404750392488350.790297894844572
DISU vs. SAHA0.3551271551629610.2259694853120710.60361327776289
DMSO vs. AZA0.1091817997722680.5195761419597941
DMSO vs. CD30.930428562197430.004010791390672840.011376872961869
DMSO vs. DISU0.4499200276587930.06707475689999330.486671442697592
DMSO vs. IL7-0.2390787220080920.1879737595127250.699129415564804
DMSO vs. SAHA-0.1043596265604350.6596758184018480.892858038539224
HIV vs. Mock in Activation0.3178410318365370.6112655847799670.999983755607037
HIV vs. Mock in Latency0.1394255310681760.4024476396683110.999834320637052
IL7 vs. CD30.7001950256063130.03060471197615330.0739132726864692
SAHA vs. CD30.8189843836371730.02173886472993540.0494343373092436
SAHA vs. IL70.1445896525860240.5550288816833410.766795469021601
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.099866 0.609524
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found