Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001518
UniProt IDQ92622
Primary gene name(s)RUBCN
Synonym gene name(s)KIAA0226
Protein nameRun domain Beclin-1-interacting and cysteine-rich domain-containing protein
Protein functionInhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II, PI3KC3-C2 function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex, possibly the HOPS complex and negatively regulates Rab7 activation, PubMed:20974968, PubMed:21062745. {ECO:0000269|PubMed:20974968, ECO:0000269|PubMed:21062745}.; FUNCTION: Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines, PubMed:22423966. Following fungal or viral infection, implicating CLEC7A, dectin-1-mediated myeloid cell activation or DDX58/RIG-I-dependent sensing of RNA viruses negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes, PubMed:22423967. {ECO:0000269|PubMed:22423966, ECO:0000269|PubMed:22423967}.
Subcellular locationLate endosome {ECO:0000269|PubMed:19270696}. Lysosome {ECO:0000269|PubMed:19270696}. Early endosome {ECO:0000269|PubMed:19270696, ECO:0000269|PubMed:20974968}. Note=Predominantly located in late endosomes/lysosomes, only partially detected in early endososome and not at all in the Golgi apparatus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q92622
Gene Ontology
(Biological Process)
Complete annatation
autophagosome maturation [GO:0097352];
immune system process [GO:0002376];
multivesicular body sorting pathway [GO:0071985];
negative regulation of autophagosome maturation [GO:1901097];
negative regulation of autophagy [GO:0010507];
negative regulation of endocytosis [GO:0045806];
negative regulation of phosphatidylinositol 3-kinase activity [GO:0043553];
phagocytosis [GO:0006909]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
Protein-protein interaction115062
Phylogenetic treeQ92622
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6566189363748280.04809557916117010.0966528758822875
AZA vs. DISU0.3440415795947990.1755479206223980.76025598363942
AZA vs. IL7-0.1097596607452060.5696244615144840.999311006273513
AZA vs. SAHA0.1268215938738770.6041757236836930.869431119909436
DISU vs. CD30.9884948312888080.00702375135720190.021607352644584
DISU vs. IL7-0.4628939685268760.06723762288734610.344179003404667
DISU vs. SAHA-0.2155842849772710.462444083463210.800119649301005
DMSO vs. AZA-0.03980854791187150.8131522625026611
DMSO vs. CD30.605853014198570.06144343816816660.11447506752454
DMSO vs. DISU-0.3855621000376930.1154998821461410.59136370451859
DMSO vs. IL7-0.06259443093704310.7288409550804440.945329043727619
DMSO vs. SAHA0.1600393050573730.4985854713290520.813373183349618
HIV vs. Mock in Activation0.2736077485742140.6609163205639070.999983755607037
HIV vs. Mock in Latency0.1037378082948390.5315950354379860.999834320637052
IL7 vs. CD30.5537184494333580.08813718483081820.17183957922066
SAHA vs. CD30.7586857843232240.0358506902765140.0753829918329937
SAHA vs. IL70.2327465536379340.3412511148928750.590839722110918
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.20468 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found