Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001511
UniProt IDQ07666
Primary gene name(s)KHDRBS1
Synonym gene name(s)SAM68
Protein nameKH domain-containing, RNA-binding, signal transduction-associated protein 1
Protein functionRecruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element, CTE-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced, CTE-containing RNA species according to, PubMed:22253824. RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A. Can regulate CD44 alternative splicing in a Ras pathway-dependent manner, By similarity. In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S, and of SMN1, PubMed:17371836, PubMed:20186123. Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4, AS4 involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4, By similarity. {ECO:0000250|UniProtKB:Q60749, ECO:0000269|PubMed:15021911, ECO:0000269|PubMed:17371836, ECO:0000269|PubMed:20186123, ECO:0000269|PubMed:20610388, ECO:0000269|PubMed:22253824, ECO:0000269|PubMed:26758068}.; FUNCTION: Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase. {ECO:0000269|PubMed:9013542}.
Subcellular locationNucleus {ECO:0000269|PubMed:1374686}. Membrane {ECO:0000269|PubMed:1374686}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q07666
Gene Ontology
(Biological Process)
Complete annatation
cell cycle arrest [GO:0007050];
cell proliferation [GO:0008283];
cell surface receptor signaling pathway [GO:0007166];
G2/M transition of mitotic cell cycle [GO:0000086];
mRNA processing [GO:0006397];
negative regulation of transcription, DNA-templated [GO:0045892];
positive regulation of RNA export from nucleus [GO:0046833];
positive regulation of translational initiation [GO:0045948];
regulation of mRNA splicing, via spliceosome [GO:0048024];
regulation of RNA export from nucleus [GO:0046831];
signal transduction [GO:0007165];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
identical protein binding [GO:0042802];
poly(A binding [GO:0008143];
poly(A RNA binding [GO:0044822];
poly(U RNA binding [GO:0008266];
RNA binding [GO:0003723];
SH3/SH2 adaptor activity [GO:0005070]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
Grb2-Sos complex [GO:0070618];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction115900
Phylogenetic treeQ07666
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7158634372643070.02965183776598760.0644337512700353
AZA vs. DISU-0.1969012686285970.435775474063960.916801046202391
AZA vs. IL70.08381727682627430.6624032019891830.999311006273513
AZA vs. SAHA0.05637916721949230.8173408036542010.95403663362155
DISU vs. CD3-0.9248541986698040.01134942755296130.0322425031134814
DISU vs. IL70.2716179252823760.2805963851570380.66771752269019
DISU vs. SAHA0.2541605833011520.3840652603223410.750648105085981
DMSO vs. AZA-0.1328527178419320.4268987662827391
DMSO vs. CD3-0.8587330795195810.007647255328027460.0197642651426889
DMSO vs. DISU0.06264910938201790.7970522926166780.975613510001278
DMSO vs. IL70.2236968966570930.2129035082050820.722716647686679
DMSO vs. SAHA0.181840923665410.4413345312445380.774133230957452
HIV vs. Mock in Activation0.005179141865072390.9933493476503890.999983755607037
HIV vs. Mock in Latency-0.01770414229915540.9144482381562110.999834320637052
IL7 vs. CD3-0.6241843713389650.05255028255539870.114398929725301
SAHA vs. CD3-0.6847922067835270.05538785098065670.107808850674682
SAHA vs. IL7-0.03108947495043140.8987898360879980.961392955374625
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.018335 0.928214
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.025 1.064 0.977 0.915 1.006
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2XA6 NMR - A/B=97-135.
3QHE X-ray 2.4Å B/D=365-419.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev binds 11932418
Envelope surface glycoprotein gp160; precursor inhibited by 18957126
Envelope surface glycoprotein gp120 inhibited by 18957126
Nef inhibited by 19150430
Rev inhibited by 10371501
Rev requires 15701759
HIV-1 virus replication enhanced by expression of human gene 18854154
Rev modulated by 12134041
Rev synergizes with 10962565

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found