Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001509
UniProt IDO15550
Primary gene name(s)KDM6A
Synonym gene name(s)UTX
Protein nameLysine-specific demethylase 6A
Protein functionHistone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code, PubMed:17851529, PubMed:17713478, PubMed:17761849. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27', PubMed:17851529, PubMed:17713478, PubMed:17761849. Plays a central role in regulation of posterior development, by regulating HOX gene expression, PubMed:17851529. Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A, PubMed:17761849. {ECO:0000269|PubMed:17713478, ECO:0000269|PubMed:17761849, ECO:0000269|PubMed:17851529, ECO:0000269|PubMed:18003914}.
Subcellular locationNucleus {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O15550
Gene Ontology
(Biological Process)
Complete annatation
canonical Wnt signaling pathway [GO:0060070];
cardiovascular system development [GO:0072358];
heart morphogenesis [GO:0003007];
histone H3-K4 methylation [GO:0051568];
in utero embryonic development [GO:0001701];
mesodermal cell differentiation [GO:0048333];
multicellular organism growth [GO:0035264];
neural tube closure [GO:0001843];
notochord morphogenesis [GO:0048570];
positive regulation of gene expression [GO:0010628];
respiratory system process [GO:0003016];
somite rostral/caudal axis specification [GO:0032525]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin DNA binding [GO:0031490];
dioxygenase activity [GO:0051213];
histone demethylase activity [GO:0032452];
histone demethylase activity, H3-K27 specific [GO:0071558];
metal ion binding [GO:0046872];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978]
Gene Ontology
(Cellular Component)
Complete annatation
histone methyltransferase complex [GO:0035097];
MLL3/4 complex [GO:0044666];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction113246
Phylogenetic treeO15550
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1279085474790730.6959095752623170.782800889495757
AZA vs. DISU0.2164378516795430.3939635164657010.902586488791209
AZA vs. IL7-0.1560743575930160.4176149600217280.999311006273513
AZA vs. SAHA-0.1595241467890350.513558464989470.829552111149943
DISU vs. CD30.3312145245781090.3623332763620810.496347856041623
DISU vs. IL7-0.3812448669969390.1315772024336620.47467131374922
DISU vs. SAHA-0.3750849768579060.199091818568750.570280935590532
DMSO vs. AZA-0.0370339366897790.8253310493506111
DMSO vs. CD30.07752178891375440.8083269674281520.86444564238696
DMSO vs. DISU-0.2558157340336550.2965969991905050.806755049278686
DMSO vs. IL7-0.1116448323006590.5353448135431760.896696737481162
DMSO vs. SAHA-0.1292868206928710.5837812670094920.857201504507045
HIV vs. Mock in Activation0.05084377332785510.9351335419287460.999983755607037
HIV vs. Mock in Latency0.03844646100587570.815944322377680.999834320637052
IL7 vs. CD3-0.01974758327213950.9509523013637910.970132253025816
SAHA vs. CD3-0.05699266033590250.8717624148578630.910300692343957
SAHA vs. IL7-0.008040269476889090.9736714191614010.990461587843337
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0256089 0.906
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3AVR X-ray 1.8Å A=880-1401.
3AVS X-ray 1.8Å A=880-1401.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05202 Transcriptional misregulation in cancer - Homo sapiens (human)