Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001500
UniProt IDQ8WZ19
Primary gene name(s)KCTD13
Synonym gene name(s)BACURD1, PDIP1, POLDIP1
Protein nameBTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1
Protein functionSubstrate-specific adapter of a BCR, BTB-CUL3-RBX1 E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD1 E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. {ECO:0000269|PubMed:19782033}.
Subcellular locationNucleus {ECO:0000269|PubMed:11593007}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8WZ19
Gene Ontology
(Biological Process)
Complete annatation
cell migration [GO:0016477];
DNA replication [GO:0006260];
negative regulation of Rho protein signal transduction [GO:0035024];
positive regulation of DNA replication [GO:0045740];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein homooligomerization [GO:0051260];
protein ubiquitination [GO:0016567];
stress fiber assembly [GO:0043149]
Gene Ontology
(Molecular Function)
Complete annatation
GTP-Rho binding [GO:0017049]
Gene Ontology
(Cellular Component)
Complete annatation
Cul3-RING ubiquitin ligase complex [GO:0031463];
nucleoplasm [GO:0005654]
Protein-protein interaction129001
Phylogenetic treeQ8WZ19
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.0772195546195940.8147615123095650.874151873115729
AZA vs. DISU0.01417198870420050.9557651428597820.996649735504266
AZA vs. IL7-0.09653830600121860.6225274037025290.999311006273513
AZA vs. SAHA0.09141445731286770.7114497191268020.918972149307812
DISU vs. CD3-0.07529646309550460.8365532064732110.889734215365638
DISU vs. IL7-0.1202318377860030.6365432162700480.892750396855013
DISU vs. SAHA0.07933072870807120.7874511188649860.942804474138073
DMSO vs. AZA0.0754959495354880.6604264302774171
DMSO vs. CD3-0.01228269117569710.9695329821935190.978889777946878
DMSO vs. DISU0.05976347900409980.8083262969216250.978268528696417
DMSO vs. IL7-0.1645610542759290.3696825131518150.828868397768739
DMSO vs. SAHA0.009883706811483690.9669462726560450.992907123593887
HIV vs. Mock in Activation0.1947696820778110.7550377280158870.999983755607037
HIV vs. Mock in Latency0.09496525427979550.5726970834762830.999834320637052
IL7 vs. CD3-0.1682704346514240.6028012224605030.719446415465214
SAHA vs. CD3-0.009077089250640690.9795720587248830.986511021450414
SAHA vs. IL70.185168326807970.4526337762417010.690841049724825
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.195808 0.952709
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4UIJ X-ray 2.7Å A/B/C/D=27-144.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
integrase interacts with 25011556

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found