Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001492
UniProt IDQ92830
Primary gene name(s)KAT2A
Synonym gene name(s)GCN5, GCN5L2, HGCN5
Protein nameHistone acetyltransferase KAT2A
Protein functionFunctions as a histone acetyltransferase, HAT to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. Also acetylates non-histone proteins, such as CEBPB, PubMed:17301242. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. {ECO:0000269|PubMed:17301242, ECO:0000269|PubMed:19103755}.
Subcellular locationNucleus {ECO:0000269|PubMed:11564863}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q92830
Gene Ontology
(Biological Process)
Complete annatation
alpha-tubulin acetylation [GO:0071929];
cardiac muscle cell differentiation [GO:0055007];
cell proliferation [GO:0008283];
cellular response to nerve growth factor stimulus [GO:1990090];
cellular response to tumor necrosis factor [GO:0071356];
chromatin remodeling [GO:0006338];
histone deubiquitination [GO:0016578];
histone H3 acetylation [GO:0043966];
histone H3-K14 acetylation [GO:0044154];
intracellular distribution of mitochondria [GO:0048312];
in utero embryonic development [GO:0001701];
metencephalon development [GO:0022037];
midbrain development [GO:0030901];
multicellular organism growth [GO:0035264];
neural tube closure [GO:0001843];
positive regulation of cell projection organization [GO:0031346];
positive regulation of gene expression, epigenetic [GO:0045815];
positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter [GO:0035948];
positive regulation of histone acetylation [GO:0035066];
positive regulation of protein targeting to mitochondrion [GO:1903955];
positive regulation of transcription regulatory region DNA binding [GO:2000679];
regulation of mitophagy [GO:1903146];
regulation of protein stability [GO:0031647];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
response to nutrient levels [GO:0031667];
response to organic cyclic compound [GO:0014070];
somitogenesis [GO:0001756];
telencephalon development [GO:0021537];
transcription from RNA polymerase II promoter [GO:0006366];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
H3 histone acetyltransferase activity [GO:0010484];
histone acetyltransferase activity [GO:0004402];
histone acetyltransferase activity, H4-K12 specific [GO:0043997];
histone deacetylase binding [GO:0042826];
transcription coactivator activity [GO:0003713];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
Ada2/Gcn5/Ada3 transcription activator complex [GO:0005671];
extracellular space [GO:0005615];
mitotic spindle [GO:0072686];
nuclear chromatin [GO:0000790];
nucleoplasm [GO:0005654];
STAGA complex [GO:0030914];
transcription factor TFTC complex [GO:0033276]
Protein-protein interaction108918
Phylogenetic treeQ92830
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4122395327504730.20848802633410.315093899701558
AZA vs. DISU-0.4363122213942220.08564400331891940.612788228525709
AZA vs. IL70.1288284857920650.5037060529974010.999311006273513
AZA vs. SAHA-0.5970891133001780.09295400394404430.383677068853374
DISU vs. CD3-0.8603277595452360.01839000690189460.0479027877081679
DISU vs. IL70.5554721642161080.02804535466442560.215157110211815
DISU vs. SAHA-0.1582338918467680.6529089367149860.892689391282925
DMSO vs. AZA0.1270068122292520.4497375630154121
DMSO vs. CD3-0.2976392215557550.3519608940533260.466302935536265
DMSO vs. DISU0.5614572661060840.02196075767407460.306043349014401
DMSO vs. IL70.009306678385394210.9587896395297250.992677490627355
DMSO vs. SAHA-0.7284176725393960.02933441864765020.18170034865613
HIV vs. Mock in Activation0.2862350979339350.6463033409882110.999983755607037
HIV vs. Mock in Latency0.06329359175473620.7019150834460150.999834320637052
IL7 vs. CD3-0.2783744682436280.3861910335347540.522814903188294
SAHA vs. CD3-1.031462820552470.004495216660729940.0130475568330509
SAHA vs. IL7-0.727711627996940.03517812686287850.144804774530593
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.333108 0.0255755
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01992 Coenzyme A nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1F68 NMR - A=730-832.
1Z4R X-ray 1.7Å A=497-662.
3D7C X-ray 2.0Å A/B=729-837.
5H84 X-ray 2.0Å A=497-662.
5H86 X-ray 2.0Å A=497-662.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat inhibits 12154097
Tat recruits 14657027
Tat enhanced by 11384967
Tat acetylated by 11384967
integrase binds 20226045
Tat binds 11384967
integrase acetylated by 20226045
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04330 Notch signaling pathway - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)