Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001489
UniProt IDP05412
Primary gene name(s)JUN
Synonym gene name(s)unknown
Protein nameTranscription factor AP-1
Protein functionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer, CRC cells, PubMed:24623306. Binds to the USP28 promoter in colorectal cancer, CRC cells, PubMed:24623306. {ECO:0000269|PubMed:10995748, ECO:0000269|PubMed:17210646, ECO:0000269|PubMed:24623306}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P05412
Gene Ontology
(Biological Process)
Complete annatation
aging [GO:0007568];
angiogenesis [GO:0001525];
axon regeneration [GO:0031103];
cellular response to calcium ion [GO:0071277];
cellular response to hormone stimulus [GO:0032870];
cellular response to potassium ion starvation [GO:0051365];
circadian rhythm [GO:0007623];
eyelid development in camera-type eye [GO:0061029];
Fc-epsilon receptor signaling pathway [GO:0038095];
leading edge cell differentiation [GO:0035026];
learning [GO:0007612];
liver development [GO:0001889];
membrane depolarization [GO:0051899];
microglial cell activation [GO:0001774];
monocyte differentiation [GO:0030224];
negative regulation by host of viral transcription [GO:0043922];
negative regulation of cell proliferation [GO:0008285];
negative regulation of DNA binding [GO:0043392];
negative regulation of neuron apoptotic process [GO:0043524];
negative regulation of protein autophosphorylation [GO:0031953];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress [GO:1990441];
outflow tract morphogenesis [GO:0003151];
positive regulation by host of viral transcription [GO:0043923];
positive regulation of cell differentiation [GO:0045597];
positive regulation of DNA replication [GO:0045740];
positive regulation of DNA-templated transcription, initiation [GO:2000144];
positive regulation of endothelial cell proliferation [GO:0001938];
positive regulation of epithelial cell migration [GO:0010634];
positive regulation of ERK1 and ERK2 cascade [GO:0070374];
positive regulation of fibroblast proliferation [GO:0048146];
positive regulation of monocyte differentiation [GO:0045657];
positive regulation of neuron apoptotic process [GO:0043525];
positive regulation of pri-miRNA transcription from RNA polymerase II promoter [GO:1902895];
positive regulation of smooth muscle cell proliferation [GO:0048661];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
Ras protein signal transduction [GO:0007265];
regulation of cell cycle [GO:0051726];
regulation of cell death [GO:0010941];
regulation of cell proliferation [GO:0042127];
regulation of sequence-specific DNA binding transcription factor activity [GO:0051090];
release of cytochrome c from mitochondria [GO:0001836];
response to cAMP [GO:0051591];
response to cytokine [GO:0034097];
response to drug [GO:0042493];
response to hydrogen peroxide [GO:0042542];
response to lipopolysaccharide [GO:0032496];
response to mechanical stimulus [GO:0009612];
response to muscle stretch [GO:0035994];
response to radiation [GO:0009314];
SMAD protein import into nucleus [GO:0007184];
SMAD protein signal transduction [GO:0060395];
transforming growth factor beta receptor signaling pathway [GO:0007179]
Gene Ontology
(Molecular Function)
Complete annatation
cAMP response element binding [GO:0035497];
chromatin binding [GO:0003682];
DNA binding [GO:0003677];
enzyme binding [GO:0019899];
GTPase activator activity [GO:0005096];
identical protein binding [GO:0042802];
poly(A RNA binding [GO:0044822];
RNA polymerase II activating transcription factor binding [GO:0001102];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
RNA polymerase II distal enhancer sequence-specific DNA binding [GO:0000980];
RNA polymerase II transcription factor activity, sequence-specific DNA binding [GO:0000981];
R-SMAD binding [GO:0070412];
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0001077];
transcriptional activator activity, RNA polymerase II transcription factor binding [GO:0001190];
transcription coactivator activity [GO:0003713];
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0000982];
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding [GO:0003705];
transcription factor activity, sequence-specific DNA binding [GO:0003700];
transcription factor binding [GO:0008134];
transcription regulatory region DNA binding [GO:0044212]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
nuclear chromosome [GO:0000228];
nuclear euchromatin [GO:0005719];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
transcriptional repressor complex [GO:0017053];
transcription factor complex [GO:0005667]
Protein-protein interaction109928
Phylogenetic treeP05412
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.435440353492710.006733378371461260.0186519902250807
AZA vs. DISU-0.2368953341690990.3956347893315240.902586488791209
AZA vs. IL7-0.6026792437752070.05022000483248670.655949855147376
AZA vs. SAHA0.1411246870978880.6590799110855880.897305868831662
DISU vs. CD3-1.685000429592720.001228440869286310.00494591817938616
DISU vs. IL7-0.3764380080043780.1776918446130970.545729829110045
DISU vs. SAHA0.3817537897866270.1919944120033820.560815943903896
DMSO vs. AZA-0.04836661042201620.8755934717006871
DMSO vs. CD3-1.48883262828950.005887692410169620.0157741572397662
DMSO vs. DISU0.1882540452213420.5136411485904050.912102132233928
DMSO vs. IL7-0.5477033417216140.08132748335740190.534442812385717
DMSO vs. SAHA0.1833550704605120.5758616504515260.85294894255264
HIV vs. Mock in Activation0.8835924460806530.2997416262180330.999983755607037
HIV vs. Mock in Latency-0.1178017863536490.6241346055674450.999834320637052
IL7 vs. CD3-2.035542644542270.0001543600187962070.000851012642031904
SAHA vs. CD3-1.316120330075810.01396046862113640.0339658156474856
SAHA vs. IL70.7432352995448540.02184696294091150.106335524498321
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
1.11 0.03814

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 0.98 6.44E-09 4.17E-07
Infected vs. Bystander 1.075 1.14E-09 6.25E-08
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-2.2 0.02389322 -2.4 0.009171654 -1.4 0.37346799
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.40324 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00570 Vinblastine approved unknown other/unknown
DB01029 Irbesartan approved, investigational unknown other/unknown
DB01169 Arsenic trioxide approved, investigational yes inducer
DB05785 LGD-1550 investigational unknown unknown
DB00852 Pseudoephedrine approved unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1A02 X-ray 2.7Å J=253-308.
1FOS X-ray 3.0Å F/H=254-315.
1JNM X-ray 2.2Å A/B=254-315.
1JUN NMR - A/B=276-314.
1S9K X-ray 3.1Å E=257-308.
1T2K X-ray 3.0Å C=254-314.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat enhances 16690925
21607373
22077140
Envelope surface glycoprotein gp120 downregulates 16179804
Envelope surface glycoprotein gp120 interacts with 16001969
Nef interacts with 24658403
Vpr regulated by 24225433
Tat activates 10644332
1248541321034562
22450687
25064159
9129988
9223324
9621077
9670954
10509564
10799874
11260070
11448159
15258149
Tat induces 26031809
Envelope surface glycoprotein gp160; precursor activates 7642615
Tat interacts with 24244375
Envelope surface glycoprotein gp120 inhibits 7957556
Nef increases phosphorylation of 26075907
Vpr involves 26270987
26270987
Tat enhanced by 24447950
Tat induces phosphorylation of 26031809
Nef downregulates 8480425
20012528
24187576
Nef enhanced by 26075907
Vpu activates 24551192
Tat binds 7690421
Envelope surface glycoprotein gp120 upregulates 10027715
10581001
12089333
1277541912089333
1277541924162774
Tat downregulates 11254713
23762317
Envelope surface glycoprotein gp120 activates 8621941
Nef activates 10388555
12419805
Vpr activates 16243842
Tat requires 18097055

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01522 Endocrine resistance - Homo sapiens (human)
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04012 ErbB signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04210 Apoptosis - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04380 Osteoclast differentiation - Homo sapiens (human)
hsa04510 Focal adhesion - Homo sapiens (human)
hsa04530 Tight junction - Homo sapiens (human)
hsa04620 Toll-like receptor signaling pathway - Homo sapiens (human)
hsa04621 NOD-like receptor signaling pathway - Homo sapiens (human)
hsa04657 IL-17 signaling pathway - Homo sapiens (human)
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
hsa04659 Th17 cell differentiation - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa04662 B cell receptor signaling pathway - Homo sapiens (human)
hsa04668 TNF signaling pathway - Homo sapiens (human)
hsa04722 Neurotrophin signaling pathway - Homo sapiens (human)
hsa04912 GnRH signaling pathway - Homo sapiens (human)
hsa04915 Estrogen signaling pathway - Homo sapiens (human)
hsa04921 Oxytocin signaling pathway - Homo sapiens (human)
hsa04932 Non-alcoholic fatty liver disease (NAFLD) - Homo sapiens (human)
hsa04933 AGE-RAGE signaling pathway in diabetic complications - Homo sapiens (human)
hsa05030 Cocaine addiction - Homo sapiens (human)
hsa05031 Amphetamine addiction - Homo sapiens (human)
hsa05120 Epithelial cell signaling in Helicobacter pylori infection - Homo sapiens (human)
hsa05132 Salmonella infection - Homo sapiens (human)
hsa05133 Pertussis - Homo sapiens (human)
hsa05140 Leishmaniasis - Homo sapiens (human)
hsa05142 Chagas disease (American trypanosomiasis) - Homo sapiens (human)
hsa05161 Hepatitis B - Homo sapiens (human)
hsa05164 Influenza A - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05168 Herpes simplex infection - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
hsa05210 Colorectal cancer - Homo sapiens (human)
hsa05211 Renal cell carcinoma - Homo sapiens (human)
hsa05224 Breast cancer - Homo sapiens (human)
hsa05231 Choline metabolism in cancer - Homo sapiens (human)
hsa05321 Inflammatory bowel disease (IBD) - Homo sapiens (human)
hsa05323 Rheumatoid arthritis - Homo sapiens (human)
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