Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001482
UniProt IDQ6ZMT4
Primary gene name(s)KDM7A
Synonym gene name(s)JHDM1D, KDM7, KIAA1718
Protein nameLysine-specific demethylase 7A
Protein functionHistone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27', H3K9me2 and H3K27me2, respectively of histone H3 and monomethylated histone H4 'Lys-20' residue, H4K20Me1, thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3, H3K4me3, affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. {ECO:0000269|PubMed:20023638, ECO:0000269|PubMed:20194436, ECO:0000269|PubMed:20622853}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q6ZMT4
Gene Ontology
(Biological Process)
Complete annatation
histone H3-K27 demethylation [GO:0071557];
histone H3-K36 demethylation [GO:0070544];
histone H3-K9 demethylation [GO:0033169];
histone H4-K20 demethylation [GO:0035574];
midbrain development [GO:0030901];
positive regulation of transcription, DNA-templated [GO:0045893];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
Protein-protein interactionunknown
Phylogenetic treeQ6ZMT4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.510917396372530.01495183505808240.0366608195860449
AZA vs. DISU0.5427466081403130.1657417013447280.747228926355439
AZA vs. IL7-0.5498539888314220.1059980575226860.850297350207259
AZA vs. SAHA0.07131281088685530.8251256238115260.957574791659228
DISU vs. CD32.039179954651010.002514207155671190.009123533621711
DISU vs. IL7-1.100062663032870.003464908565959050.0602521520309614
DISU vs. SAHA-0.4720136969613940.189363995772420.5567620346836
DMSO vs. AZA-0.02168085397273920.9460766574831681
DMSO vs. CD31.471098202812690.01307441654410670.0312992349017904
DMSO vs. DISU-0.5680568530859290.108137494179230.581806680424036
DMSO vs. IL7-0.5200725092262830.07907555320204410.528568531921846
DMSO vs. SAHA0.08571410642072260.7569446698264520.929437275456612
HIV vs. Mock in Activation0.01107455724168820.9927366903080640.999983755607037
HIV vs. Mock in Latency-0.1135018391362320.6200650566623640.999834320637052
IL7 vs. CD30.9721339036582960.1058602146840040.198165799296075
SAHA vs. CD31.55460639353570.009360321191574260.0242537896074663
SAHA vs. IL70.6145813664444630.04033596804500720.15850479205173
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.586292 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3KV5 X-ray 2.3Å A/D=1-488.
3KV6 X-ray 2.8Å A/D=1-488.
3KV9 X-ray 2.2Å A=92-488.
3KVA X-ray 2.7Å A=92-488.
3KVB X-ray 2.6Å A=92-488.
3U78 X-ray 2.6Å A=92-488.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found