Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001476
UniProt IDQ96ST2
Primary gene name(s)IWS1
Synonym gene name(s)IWS1L
Protein nameProtein IWS1 homolog
Protein functionTranscription factor which plays a key role in defining the composition of the RNA polymerase II, RNAPII elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain, CTD of the RNAPII subunit RPB1, POLR2A. The SUPT6H:IWS1:CTD complex recruits mRNA export factors, ALYREF/THOC4, EXOSC10 as well as histone modifying enzymes, such as SETD2 to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. {ECO:0000269|PubMed:17184735, ECO:0000269|PubMed:17234882, ECO:0000269|PubMed:19141475}.
Subcellular locationNucleus {ECO:0000255|PROSITE-ProRule:PRU00649, ECO:0000269|PubMed:17184735, ECO:0000269|PubMed:17234882}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96ST2
Gene Ontology
(Biological Process)
Complete annatation
mRNA processing [GO:0006397];
mRNA transport [GO:0051028];
regulation of DNA-templated transcription, elongation [GO:0032784];
regulation of histone H3-K36 trimethylation [GO:2001253];
regulation of histone H4 acetylation [GO:0090239];
regulation of mRNA export from nucleus [GO:0010793];
regulation of mRNA processing [GO:0050684];
RNA splicing [GO:0008380];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677]
Gene Ontology
(Cellular Component)
Complete annatation
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction120807
Phylogenetic treeQ96ST2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7737268053508180.01874151026770490.044136256680445
AZA vs. DISU-0.2524093946003290.3197326601191350.870739370249409
AZA vs. IL70.1900081919033240.3243799831740820.999311006273513
AZA vs. SAHA-0.1043142069922470.6695179341722140.901985283735092
DISU vs. CD3-1.038664627219320.004545148623416480.0149625617709937
DISU vs. IL70.4333046743296380.0864821085748350.389044232816993
DISU vs. SAHA0.1493937237587140.6091834890148450.877213385767783
DMSO vs. AZA-0.02545744353722170.8797629798001161
DMSO vs. CD3-0.8094188904599010.0118561485395040.0288317801045404
DMSO vs. DISU0.2253799011668640.3571346389920050.845695250833134
DMSO vs. IL70.2225914743212860.2172197362667440.726133701755938
DMSO vs. SAHA-0.08609159632735720.7154093089693940.915196816524721
HIV vs. Mock in Activation0.01083001699244990.9861054466159950.999983755607037
HIV vs. Mock in Latency-0.1146935683872950.4888467129962070.999834320637052
IL7 vs. CD3-0.5760066396346390.07366804088618780.149608898197177
SAHA vs. CD3-0.9030775134367970.01118188119733070.0282452144894021
SAHA vs. IL7-0.2976131352330950.2226310620545610.4624418670815
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.138783 0.413468
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.013 1.026 0.949 0.963 1.037
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
integrase binds 26245978
integrase overlaps 26245978
integrase displaces 26245978
HIV-1 virus replication inhibited by expression of human gene 25590759

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found