Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001475
UniProt IDQ9Y6Y0
Primary gene name(s)IVNS1ABP
Synonym gene name(s)ARA3, FLARA3, KIAA0850, NS1, NS1BP
Protein nameInfluenza virus NS1A-binding protein
Protein functionPlays a role in cell division and in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats. Protects cells from cell death induced by actin destabilization; Protects neurons from dendritic spines and actin filaments damage induced by the actin-destabilizing cytochalasin B when overexpressed. Activates Erk signaling pathway when overexpressed in cultured cell lines, By similarity. May be a component of the cellular splicing machinery with a role in pre-mRNA splicing; may mediate the inhibition of splicing by NS/influenza virus NS1A protein. Functions as modifier of the AHR/Aryl hydrocarbon receptor pathway increasing the concentration of AHR available to activate transcription. {ECO:0000250, ECO:0000269|PubMed:16582008}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:9696811}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:9696811}. Nucleus, nucleoplasm {ECO:0000269|PubMed:9696811}. Note=Associated with actin filaments, By similarity. Localization related to speckle domains which correspond to interchromatin granules and are enriched in factors involved in pre-mRNA splicing. Following influenza A virus infection, redistribution from speckles to a more diffuse distribution in the nucleoplasm. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y6Y0
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of intrinsic apoptotic signaling pathway [GO:2001243];
response to virus [GO:0009615];
RNA splicing [GO:0008380];
transcription from RNA polymerase III promoter [GO:0006383]
Gene Ontology
(Molecular Function)
Complete annatation
unknown
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
cytoplasm [GO:0005737];
nucleoplasm [GO:0005654];
spliceosomal complex [GO:0005681];
transcription factor complex [GO:0005667]
Protein-protein interaction115869
Phylogenetic treeQ9Y6Y0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.05060555165989330.8771017856991060.919373956050972
AZA vs. DISU-0.1319533173624650.6036599479882130.955158276076142
AZA vs. IL7-0.142496622022390.4582352718829210.999311006273513
AZA vs. SAHA0.02388311982313020.9219683640859090.982071981797342
DISU vs. CD3-0.1954889408703690.5900846250274080.702879149558526
DISU vs. IL7-0.01972049212547660.9380189837177320.988617459213365
DISU vs. SAHA0.1569393944750960.5938269195734330.869818787529518
DMSO vs. AZA-0.01340874345234980.936117571691581
DMSO vs. CD3-0.0741299043596650.8165707084058260.870960861210881
DMSO vs. DISU0.1170764207232510.6330371615068190.94583860514751
DMSO vs. IL7-0.1219753724954080.4971702182758540.885687081722074
DMSO vs. SAHA0.0301526961336250.8981672580394790.975026503245809
HIV vs. Mock in Activation0.06295145724688990.9193454595558160.999983755607037
HIV vs. Mock in Latency0.2229268352614780.17650891517780.999834320637052
IL7 vs. CD3-0.185761430949880.5632079936779220.684450059558761
SAHA vs. CD3-0.0515207414733720.8842675562537120.919980122384773
SAHA vs. IL70.1627533806131960.5036331376419770.731383999693175
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.37654 0.00420274
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.87 1.112 0.725 0.651 1.232
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05164 Influenza A - Homo sapiens (human)
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