Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001474
UniProt IDQ9NZM3
Primary gene name(s)ITSN2
Synonym gene name(s)KIAA1256, SH3D1B, SWAP
Protein nameIntersectin-2
Protein functionAdapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles, CCPs. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1, ITGB1 and transferrin receptor, TFR. Plays a role in dendrite formation by melanocytes, PubMed:23999003. {ECO:0000269|PubMed:19458185, ECO:0000269|PubMed:22648170, ECO:0000269|PubMed:23999003}.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NZM3
Gene Ontology
(Biological Process)
Complete annatation
endocytosis [GO:0006897];
positive regulation of dendrite extension [GO:1903861];
regulation of Rho protein signal transduction [GO:0035023]
Gene Ontology
(Molecular Function)
Complete annatation
calcium ion binding [GO:0005509];
Rho guanyl-nucleotide exchange factor activity [GO:0005089];
SH3/SH2 adaptor activity [GO:0005070]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062]
Protein-protein interaction119098
Phylogenetic treeQ9NZM3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6215285265427230.05956893923542010.115292260941157
AZA vs. DISU0.1565320450528090.5356266377305420.94117888998271
AZA vs. IL70.01891815657272080.9214732207778950.999311006273513
AZA vs. SAHA-0.3836975987504810.1165843231503080.435289284980489
DISU vs. CD30.7652728691430460.03590332086471390.0827406602529732
DISU vs. IL7-0.1463928467354370.5606503983047470.857872165643804
DISU vs. SAHA-0.539406844303960.0646732159725730.321931503192975
DMSO vs. AZA-0.02851231431856340.8645798362081981
DMSO vs. CD30.5819841231089020.07059114448813030.128336415628585
DMSO vs. DISU-0.1867865372490750.4434876345247450.884953831766437
DMSO vs. IL70.05460190034941630.7609608616135810.951841190064606
DMSO vs. SAHA-0.3626066386560180.1247162693490250.421426725271243
HIV vs. Mock in Activation0.1496211990857560.8115176693936190.999983755607037
HIV vs. Mock in Latency0.02019646748875080.9023702447978570.999834320637052
IL7 vs. CD30.6488350928205460.04476690460769460.100010605180344
SAHA vs. CD30.2124689867454680.547468551983980.651943980908619
SAHA vs. IL7-0.4067758205822950.09537693014412110.279710475679471
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.130311 0.429417
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.975 1.013 1.037 1.139 0.924
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
209898_x_at 1.32 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1J3T NMR - A=898-958.
1UDL NMR - A=1103-1187.
1UE9 NMR - A=1056-1122.
1UFF NMR - A=762-842.
1UHF NMR - A=983-1038.
3GF9 X-ray 2.5Å A=1130-1406.
3JZY X-ray 1.5Å A=1201-1692.
4IIO X-ray 1.7Å A/B=901-955.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 19460752

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found