Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001466
UniProt IDP08648
Primary gene name(s)ITGA5
Synonym gene name(s)FNRA
Protein nameIntegrin alpha-5
Protein functionIntegrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site, site 2 which is distinct from the classical ligand-binding site, site 1 and this induces integrin conformational changes and enhanced ligand binding to site 1, PubMed:18635536, PubMed:25398877. ITGA5:ITGB1 acts as a receptor for fibrillin-1, FBN1 and mediates R-G-D-dependent cell adhesion to FBN1, PubMed:12807887, PubMed:17158881. {ECO:0000269|PubMed:12807887, ECO:0000269|PubMed:17158881, ECO:0000269|PubMed:18635536, ECO:0000269|PubMed:25398877}.; FUNCTION:, Microbial infection Integrin ITGA5:ITGB1 acts as a receptor for human metapneumovirus, PubMed:12907437. Integrin ITGA2:ITGB1 acts as a receptor for human parvovirus B19, PubMed:24478423. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions, PubMed:10397733. {ECO:0000269|PubMed:10397733, ECO:0000269|PubMed:12907437, ECO:0000269|PubMed:24478423}.
Subcellular locationMembrane;
Single-pass type I membrane protein. Cell junction, focal adhesion {ECO:0000269|PubMed:17158881}. Cell surface {ECO:0000269|PubMed:17158881}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P08648
Gene Ontology
(Biological Process)
Complete annatation
angiogenesis [GO:0001525];
cell adhesion [GO:0007155];
cell adhesion mediated by integrin [GO:0033627];
cell-cell adhesion mediated by integrin [GO:0033631];
cell-substrate adhesion [GO:0031589];
cell-substrate junction assembly [GO:0007044];
endodermal cell differentiation [GO:0035987];
extracellular matrix organization [GO:0030198];
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules [GO:0007157];
heterotypic cell-cell adhesion [GO:0034113];
integrin-mediated signaling pathway [GO:0007229];
leukocyte cell-cell adhesion [GO:0007159];
leukocyte migration [GO:0050900];
memory [GO:0007613];
negative regulation of anoikis [GO:2000811];
positive regulation of cell migration [GO:0030335];
positive regulation of cell-substrate adhesion [GO:0010811];
positive regulation of peptidyl-tyrosine phosphorylation [GO:0050731];
positive regulation of sprouting angiogenesis [GO:1903672];
positive regulation of vascular endothelial growth factor receptor signaling pathway [GO:0030949];
wound healing, spreading of epidermal cells [GO:0035313]
Gene Ontology
(Molecular Function)
Complete annatation
metal ion binding [GO:0046872];
platelet-derived growth factor receptor binding [GO:0005161];
vascular endothelial growth factor receptor 2 binding [GO:0043184];
virus receptor activity [GO:0001618]
Gene Ontology
(Cellular Component)
Complete annatation
alphav-beta3 integrin-vitronectin complex [GO:0071062];
cell surface [GO:0009986];
cytoplasmic vesicle [GO:0031410];
endoplasmic reticulum [GO:0005783];
external side of plasma membrane [GO:0009897];
focal adhesion [GO:0005925];
Golgi apparatus [GO:0005794];
integrin complex [GO:0008305];
plasma membrane [GO:0005886];
ruffle [GO:0001726];
synapse [GO:0045202]
Protein-protein interaction109884
Phylogenetic treeP08648
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.509657825891444.25992574548673e-131.09193198700639e-11
AZA vs. DISU0.178605686315890.4797834535106560.931225724042767
AZA vs. IL7-0.4343569361302810.02408630802385410.495297299922992
AZA vs. SAHA-0.3149342750884780.1985370470128910.566228589651869
DISU vs. CD32.675127449472754.16444656536896e-121.3617107923291e-10
DISU vs. IL7-0.6218608188959850.01383961348992150.143587180564739
DISU vs. SAHA-0.491831101288070.09260439345859530.388872466110566
DMSO vs. AZA0.09149585506585170.584654415461211
DMSO vs. CD32.584709984942422.59792187762287e-147.20767828478609e-13
DMSO vs. DISU-0.09025608459064160.7110832746700660.962004276809724
DMSO vs. IL7-0.5178317949295390.00406124364694560.128082108739968
DMSO vs. SAHA-0.4112415297803240.08210525440160530.33425399489103
HIV vs. Mock in Activation-0.07270048026903430.9072541672569280.999983755607037
HIV vs. Mock in Latency0.0111190022162640.9463678693382690.999834320637052
IL7 vs. CD32.082675939021064.86907514307688e-109.23164397881734e-09
SAHA vs. CD32.170309503109064.89873486220205e-096.93182482379201e-08
SAHA vs. IL70.115411862791350.6365338421240970.820936955525209
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.153344 0.307202
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.961 1.184 1.048 1.108 1.026
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3VI3 X-ray 2.9Å A/C=42-664.
3VI4 X-ray 2.9Å A/C=42-664.
4WJK X-ray 1.8Å A=42-491.
4WK0 X-ray 1.7Å A=42-491.
4WK2 X-ray 2.5Å A=42-491.
4WK4 X-ray 2.5Å A=42-491.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 interacts with 16473416
Tat activates 8757349
Vpr upregulates 10713718
Tat upregulates 8599839
Tat binds 2202737
Tat interacts with 25313583
Envelope surface glycoprotein gp120 inhibits 22241990

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04145 Phagosome - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04510 Focal adhesion - Homo sapiens (human)
hsa04512 ECM-receptor interaction - Homo sapiens (human)
hsa04640 Hematopoietic cell lineage - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05131 Shigellosis - Homo sapiens (human)
hsa05133 Pertussis - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)
hsa05410 Hypertrophic cardiomyopathy (HCM) - Homo sapiens (human)
hsa05412 Arrhythmogenic right ventricular cardiomyopathy (ARVC) - Homo sapiens (human)
hsa05414 Dilated cardiomyopathy - Homo sapiens (human)