Protein Information (annotations from UniProt)
| Database ID | HIV0001460 |
| UniProt ID | Q9H9L3 |
| Primary gene name(s) | ISG20L2 |
| Synonym gene name(s) | unknown |
| Protein name | Interferon-stimulated 20 kDa exonuclease-like 2 |
| Protein function | 3'-> 5'-exoribonuclease involved in ribosome biogenesis in the processing of the 12S pre-rRNA. Displays a strong specificity for a 3'-end containing a free hydroxyl group. {ECO:0000269|PubMed:18065403}. |
| Subcellular location | Nucleus, nucleolus {ECO:0000269|PubMed:12429849, ECO:0000269|PubMed:18065403}. |
| ECO code | Click here for more information. |
| Amino acid sequence FASTA format: Q9H9L3 |
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| Gene Ontology (Biological Process) Complete annatation | rRNA processing [GO:0006364] |
| Gene Ontology (Molecular Function) Complete annatation | 3'-5'-exoribonuclease activity [GO:0000175]; poly(A RNA binding [GO:0044822] |
| Gene Ontology (Cellular Component) Complete annatation | nucleolus [GO:0005730]; nucleoplasm [GO:0005654] |
| Protein-protein interaction | 123618 |
| Phylogenetic tree | Q9H9L3 |
| HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is also observed Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
| Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
| Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Gene Expression Profile top
Up-regulated; 
Down-regulated
For brief introduction to each study, please go to the help page.
Gene expression during HIV latency
| (1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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| Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
| AZA vs. CD3 | 0.875203769195721 | 0.00798658684013642 | 0.0215453439868509 |
| AZA vs. DISU | 0.00390660057737987 | 0.987697695350332 | 0.998932793566471 |
| AZA vs. IL7 | 0.164053573925125 | 0.395383856064427 | 0.999311006273513 |
| AZA vs. SAHA | -0.0715794961342575 | 0.769934407167904 | 0.938844139593025 |
| DISU vs. CD3 | -0.883512009772337 | 0.0155450644999859 | 0.041851456023002 |
| DISU vs. IL7 | 0.150550215490791 | 0.550624603596745 | 0.853187984296529 |
| DISU vs. SAHA | -0.0733417084514113 | 0.801624975801249 | 0.948438947308947 |
| DMSO vs. AZA | 0.00848194049041116 | 0.959866908416289 | 1 |
| DMSO vs. CD3 | -0.878156747629673 | 0.00642700739510327 | 0.0170163209854536 |
| DMSO vs. DISU | 0.00272688295520001 | 0.99109626916469 | 0.99806609310612 |
| DMSO vs. IL7 | 0.162891889497747 | 0.366945408674233 | 0.826490667229054 |
| DMSO vs. SAHA | -0.08549100338645 | 0.717764373272579 | 0.915954857713043 |
| HIV vs. Mock in Activation | 0.196146048893803 | 0.752515240171684 | 0.999983755607037 |
| HIV vs. Mock in Latency | 0.146816139502359 | 0.375987033653463 | 0.999834320637052 |
| IL7 vs. CD3 | -0.705182837330135 | 0.028955749586734 | 0.0706001315950892 |
| SAHA vs. CD3 | -0.97024617048115 | 0.00646543124035981 | 0.0177799359109895 |
| SAHA vs. IL7 | -0.237709633067501 | 0.330630768811295 | 0.581080588674533 |
| (2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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| Log2 Fold Change | P Value | ||
| unknown | unknown | ||
Gene expression during HIV infection and replication
| (1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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| Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
| Infected vs. Mock | unknown | unknown | unknown | ||
| Infected vs. Bystander | unknown | unknown | unknown | ||
| (2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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| Log2 Fold Change | unknown | ||||
| (3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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| Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
| unknown | unknown | unknown | unknown | unknown | unknown |
| (4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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| Up-regulated (True) | FALSE | ||||
| (5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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| Test Status | Log2 Fold Change | P Value | |||
| OK | 0.159509 | 0.269935 | |||
| (6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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| Log2 Fold Change | P Value | ||||
| unknown | unknown | ||||
Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV
| (1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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| 6 h | 24 h | 48 h | 72 h | RTi | ||
| 0.867 | 0.693 | 0.513 | 0.394 | 0.74 | ||
| (2). Navare et al., Virology, 2012 SUP-T1 cell line |
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| FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
| unknown | unknown | unknown | unknown | unknown | unknown | unknown |
| (3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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| Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
| unknown | unknown | unknown | unknown | |||
Protein Overview top
Drug-protein Interaction (annotations from DrugBank) top
Protein Secondary Structure (annotations from PDB) top
HIV-1 Interaction (annotations from NCBI HIV-1 Interaction Database) top
| HIV Partner | Interaction Type | PubMed |
|---|---|---|
| HIV-1 virus replication | enhanced by expression of human gene |
18976975 |
Metabolic/Signalling Pathway (annotations from KEGG database) top
| Pathway Accession Number | Description | not found |
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