Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001457
UniProt IDQ9Y4H2
Primary gene name(s)IRS2
Synonym gene name(s)unknown
Protein nameInsulin receptor substrate 2
Protein functionMay mediate the control of various cellular processes by insulin.
Subcellular locationCytoplasm, cytosol {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y4H2
Gene Ontology
(Biological Process)
Complete annatation
brain development [GO:0007420];
cell proliferation [GO:0008283];
cellular response to glucose stimulus [GO:0071333];
cellular response to insulin stimulus [GO:0032869];
glucose metabolic process [GO:0006006];
insulin receptor signaling pathway [GO:0008286];
lipid homeostasis [GO:0055088];
mammary gland development [GO:0030879];
MAPK cascade [GO:0000165];
negative regulation of B cell apoptotic process [GO:0002903];
negative regulation of kinase activity [GO:0033673];
negative regulation of plasma membrane long-chain fatty acid transport [GO:0010748];
phosphatidylinositol-mediated signaling [GO:0048015];
positive regulation of B cell proliferation [GO:0030890];
positive regulation of cell migration [GO:0030335];
positive regulation of cell proliferation [GO:0008284];
positive regulation of fatty acid beta-oxidation [GO:0032000];
positive regulation of glucose import [GO:0046326];
positive regulation of glucose metabolic process [GO:0010907];
positive regulation of glycogen biosynthetic process [GO:0045725];
positive regulation of insulin secretion [GO:0032024];
positive regulation of mesenchymal cell proliferation [GO:0002053];
regulation of lipid metabolic process [GO:0019216];
regulation of phosphatidylinositol 3-kinase signaling [GO:0014066];
response to glucose [GO:0009749];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
1-phosphatidylinositol-3-kinase activity [GO:0016303];
insulin receptor binding [GO:0005158];
phosphatidylinositol 3-kinase binding [GO:0043548];
phosphatidylinositol-4,5-bisphosphate 3-kinase activity [GO:0046934];
Ras guanyl-nucleotide exchange factor activity [GO:0005088];
signal transducer activity [GO:0004871]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
plasma membrane [GO:0005886]
Protein-protein interaction114209
Phylogenetic treeQ9Y4H2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      Negatively associated
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.282214775257690.0001248644582038110.000588427640670666
AZA vs. DISU0.3840770478293130.1333042638953120.703531604593371
AZA vs. IL7-0.371831847260720.05677142265696820.690210880962922
AZA vs. SAHA0.1751368271547960.4756224712599460.807217611562026
DISU vs. CD31.652688931735611.03132651220195e-057.65823440637051e-05
DISU vs. IL7-0.7640190344250840.002753960305732050.0521557181016466
DISU vs. SAHA-0.2079274467790280.4797149886676360.811332577063174
DMSO vs. AZA-0.1347345635454090.4289577456431071
DMSO vs. CD31.134284068211960.0004910459293362420.00183528693387793
DMSO vs. DISU-0.521178740405290.03466908732046730.375872766848519
DMSO vs. IL7-0.2295116579962030.2087750477997940.717686982288547
DMSO vs. SAHA0.3029247898521590.2021388521125880.542456310574931
HIV vs. Mock in Activation-0.2706387134887120.666531681863210.999983755607037
HIV vs. Mock in Latency-0.4993164569957340.002908189380198210.14233132051009
IL7 vs. CD30.9196535037866370.004794110544493520.0161659123573044
SAHA vs. CD31.431563755080127.49508593732573e-050.00037252499540082
SAHA vs. IL70.5420163820638150.02767072215519140.123514499783049
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.316528 0.0344248
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
209184_s_at 1.68 No downregulated in CD8+ cells
209185_s_at 2.47 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3FQW X-ray 1.9Å C=1097-1105.
3FQX X-ray 1.7Å C=1097-1105.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04068 FoxO signaling pathway - Homo sapiens (human)
hsa04152 AMPK signaling pathway - Homo sapiens (human)
hsa04211 Longevity regulating pathway - Homo sapiens (human)
hsa04213 Longevity regulating pathway - multiple species - Homo sapiens (human)
hsa04910 Insulin signaling pathway - Homo sapiens (human)
hsa04920 Adipocytokine signaling pathway - Homo sapiens (human)
hsa04923 Regulation of lipolysis in adipocytes - Homo sapiens (human)
hsa04930 Type II diabetes mellitus - Homo sapiens (human)
hsa04931 Insulin resistance - Homo sapiens (human)
hsa04932 Non-alcoholic fatty liver disease (NAFLD) - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)
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