Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001451
UniProt IDO95373
Primary gene name(s)IPO7
Synonym gene name(s)RANBP7
Protein nameImportin-7
Protein functionFunctions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals, NLS and to promote translocation of import substrates through the nuclear pore complex, NPC by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding, BIB domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates the nuclear import of HIV-1 reverse transcription complex, RTC integrase. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. {ECO:0000269|PubMed:10228156, ECO:0000269|PubMed:12853482, ECO:0000269|PubMed:9687515}.
Subcellular locationCytoplasm. Nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95373
Gene Ontology
(Biological Process)
Complete annatation
innate immune response [GO:0045087];
protein import into nucleus [GO:0006606];
signal transduction [GO:0007165];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase regulator activity [GO:0030695];
protein transporter activity [GO:0008565];
Ran GTPase binding [GO:0008536];
transporter activity [GO:0005215]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
membrane [GO:0016020];
nuclear pore [GO:0005643];
nucleoplasm [GO:0005654]
Protein-protein interaction115782
Phylogenetic treeO95373
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.339220831259755.63724110791419e-050.000289585882680582
AZA vs. DISU0.2334963837179680.3576895421687420.892482504096917
AZA vs. IL70.3365976397208360.1592454741972950.92284007403915
AZA vs. SAHA-0.1530812255835010.5297766233111210.837755093550286
DISU vs. CD3-1.118207772791940.002323570460069590.00850753002552563
DISU vs. IL70.09458521184606140.7073499751643840.924693836642469
DISU vs. SAHA-0.386466484372220.1883773395977360.55565238213418
DMSO vs. AZA-0.06321904884960990.7405821303904851
DMSO vs. CD3-1.415526605124451.35560775212928e-057.58908613371522e-05
DMSO vs. DISU-0.2991081089795790.2219658339003980.733913231642466
DMSO vs. IL70.4073009821926320.04745518772309130.435164757353778
DMSO vs. SAHA-0.09731507484176590.6793584522699890.89968124570397
HIV vs. Mock in Activation0.02177645925048570.9721534914601490.999983755607037
HIV vs. Mock in Latency-0.1651838576828330.3157273220850680.999834320637052
IL7 vs. CD3-0.9931522070574220.002152017292545880.00820991056708279
SAHA vs. CD3-1.518441310851312.58213370816218e-050.000145413882593438
SAHA vs. IL7-0.4940836147337060.0479272640663390.178472227899208
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.584 0.01181

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -0.977313826
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.148199 0.310211
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.982 0.975 0.93 0.888 0.957
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr imported by 12853482
Gag-Pol complexes with 23125841
Nef interacts with 23439083
matrix imported by 12853482
Pol interacts with 23439083
nucleocapsid imported by 12853482
integrase binds 17360709
integrase imported by 12853482
HIV-1 virus replication enhanced by expression of human gene 23439083
Nef complexes with 23125841
Rev interacts with 22174317
Envelope surface glycoprotein gp120 complexes with 23125841
reverse transcriptase interacts with 23439083
Pr55(Gag) complexes with 23125841
reverse transcriptase imported by 12853482

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found