Database ID | HIV0001450 |
UniProt ID | O00410 |
Primary gene name(s) | IPO5 |
Synonym gene name(s) | KPNB3, RANBP5 |
Protein name | Importin-5 |
Protein function | Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals, NLS in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex, NPC through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus, By similarity. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding, BIB domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation, By similarity. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. {ECO:0000250|UniProtKB:Q8BKC5, ECO:0000269|PubMed:9687515}. |
Subcellular location | Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus. |
ECO code | Click here for more information. |
Amino acid sequence FASTA format: O00410 |
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Gene Ontology (Biological Process) Complete annatation | cellular response to amino acid stimulus [GO:0071230]; NLS-bearing protein import into nucleus [GO:0006607]; positive regulation of protein import into nucleus [GO:0042307]; protein import into nucleus, docking [GO:0000059]; protein import into nucleus, translocation [GO:0000060]; ribosomal protein import into nucleus [GO:0006610]; viral process [GO:0016032] |
Gene Ontology (Molecular Function) Complete annatation | GTPase inhibitor activity [GO:0005095]; nuclear localization sequence binding [GO:0008139]; poly(A RNA binding [GO:0044822]; protein transporter activity [GO:0008565]; Ran GTPase binding [GO:0008536] |
Gene Ontology (Cellular Component) Complete annatation | cytoplasm [GO:0005737]; Golgi apparatus [GO:0005794]; intracellular membrane-bounded organelle [GO:0043231]; membrane [GO:0016020]; nuclear membrane [GO:0031965]; nuclear periphery [GO:0034399]; nuclear pore [GO:0005643]; nucleolus [GO:0005730]; nucleoplasm [GO:0005654]; nucleus [GO:0005634] |
Protein-protein interaction | 110041 |
Phylogenetic tree | O00410 |
HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Up-regulated;
Down-regulated
For brief introduction to each study, please go to the help page.
(1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
AZA vs. CD3 | 1.57103790023576 | 2.75493497070567e-06 | 1.93577528570983e-05 |
AZA vs. DISU | -0.0811206102040843 | 0.74821993591443 | 0.976008954784031 |
AZA vs. IL7 | 0.462868711438231 | 0.0340898240842087 | 0.570633544062172 |
AZA vs. SAHA | -0.24164511670925 | 0.32155611741688 | 0.692710852405634 |
DISU vs. CD3 | -1.66408579077359 | 7.45094818255243e-06 | 5.76272924083096e-05 |
DISU vs. IL7 | 0.535479717387549 | 0.0337304230105836 | 0.239513013134502 |
DISU vs. SAHA | -0.160517643223971 | 0.581417314227709 | 0.865147684815138 |
DMSO vs. AZA | -0.0674811080262765 | 0.686609233095651 | 1 |
DMSO vs. CD3 | -1.65170454064773 | 4.72118099059671e-07 | 3.59578319632656e-06 |
DMSO vs. DISU | 0.0111431493592574 | 0.963532770035452 | 0.994294013509907 |
DMSO vs. IL7 | 0.537948562106917 | 0.00289421260748379 | 0.104129487011499 |
DMSO vs. SAHA | -0.18157611715392 | 0.440736837215297 | 0.773587821480051 |
HIV vs. Mock in Activation | -0.149335495691099 | 0.810638344993645 | 0.999983755607037 |
HIV vs. Mock in Latency | -0.15843024043856 | 0.33685708309762 | 0.999834320637052 |
IL7 vs. CD3 | -1.09853516232919 | 0.000711682351635323 | 0.00317748462268934 |
SAHA vs. CD3 | -1.8388630599771 | 4.49539305558133e-07 | 3.98035096049416e-06 |
SAHA vs. IL7 | -0.709251523625222 | 0.00370322724748096 | 0.030995251265146 |
(2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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Log2 Fold Change | P Value | ||
-0.424 | 0.03673 |
(1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
Infected vs. Mock | unknown | unknown | unknown | ||
Infected vs. Bystander | unknown | unknown | unknown | ||
(2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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Log2 Fold Change | -1.097272986 | ||||
(3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
unknown | unknown | unknown | unknown | unknown | unknown |
(4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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Up-regulated (True) | FALSE | ||||
(5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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Test Status | Log2 Fold Change | P Value | |||
OK | 0.0700554 | 0.68608 | |||
(6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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Log2 Fold Change | P Value | ||||
unknown | unknown |
(1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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6 h | 24 h | 48 h | 72 h | RTi | ||
0.971 | 1.013 | 0.97 | 0.891 | 0.919 | ||
(2). Navare et al., Virology, 2012 SUP-T1 cell line |
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FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
unknown | unknown | unknown | unknown | unknown | unknown | unknown |
(3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
unknown | unknown | unknown | unknown |
HIV Partner | Interaction Type | PubMed |
---|---|---|
Vpr | interacts with |
18445273 20015032 |
Rev | binds |
9405152 9891055 12614157 21326825 |
matrix | imported by |
8105392 9562972 10860744 20015032 |
Rev | inhibited by |
9891055 |
integrase | imported by |
1631159 9275210 9366553 10366569 10525473 10888652 11035935 11278458 11389849 12368302 12414950 20015032 225094829275210 11035935 11278458 |
integrase | binds |
9275210 |
Tat | imported by |
9430704 9891055 |
Tat | binds |
9891055 |
Vpr | regulates |
9436978 9463369 9582382 9621063 9817747 10772949 19275582 |
capsid | interacts with |
25505242 |
Pathway Accession Number | Description | not found |
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