Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001450
UniProt IDO00410
Primary gene name(s)IPO5
Synonym gene name(s)KPNB3, RANBP5
Protein nameImportin-5
Protein functionFunctions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals, NLS in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex, NPC through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus, By similarity. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding, BIB domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation, By similarity. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. {ECO:0000250|UniProtKB:Q8BKC5, ECO:0000269|PubMed:9687515}.
Subcellular locationCytoplasm. Nucleus. Nucleus, nucleolus. Note=Nucleus;
nuclear rim. Found particularly in the nuclear rim and nucleolus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O00410
Gene Ontology
(Biological Process)
Complete annatation		cellular response to amino acid stimulus [GO:0071230];
NLS-bearing protein import into nucleus [GO:0006607];
positive regulation of protein import into nucleus [GO:0042307];
protein import into nucleus, docking [GO:0000059];
protein import into nucleus, translocation [GO:0000060];
ribosomal protein import into nucleus [GO:0006610];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation		GTPase inhibitor activity [GO:0005095];
nuclear localization sequence binding [GO:0008139];
poly(A RNA binding [GO:0044822];
protein transporter activity [GO:0008565];
Ran GTPase binding [GO:0008536]
Gene Ontology
(Cellular Component)
Complete annatation		cytoplasm [GO:0005737];
Golgi apparatus [GO:0005794];
intracellular membrane-bounded organelle [GO:0043231];
membrane [GO:0016020];
nuclear membrane [GO:0031965];
nuclear periphery [GO:0034399];
nuclear pore [GO:0005643];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction110041
Phylogenetic treeO00410
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status		 Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.571037900235762.75493497070567e-061.93577528570983e-05
AZA vs. DISU-0.08112061020408430.748219935914430.976008954784031
AZA vs. IL70.4628687114382310.03408982408420870.570633544062172
AZA vs. SAHA-0.241645116709250.321556117416880.692710852405634
DISU vs. CD3-1.664085790773597.45094818255243e-065.76272924083096e-05
DISU vs. IL70.5354797173875490.03373042301058360.239513013134502
DISU vs. SAHA-0.1605176432239710.5814173142277090.865147684815138
DMSO vs. AZA-0.06748110802627650.6866092330956511
DMSO vs. CD3-1.651704540647734.72118099059671e-073.59578319632656e-06
DMSO vs. DISU0.01114314935925740.9635327700354520.994294013509907
DMSO vs. IL70.5379485621069170.002894212607483790.104129487011499
DMSO vs. SAHA-0.181576117153920.4407368372152970.773587821480051
HIV vs. Mock in Activation-0.1493354956910990.8106383449936450.999983755607037
HIV vs. Mock in Latency-0.158430240438560.336857083097620.999834320637052
IL7 vs. CD3-1.098535162329190.0007116823516353230.00317748462268934
SAHA vs. CD3-1.83886305997714.49539305558133e-073.98035096049416e-06
SAHA vs. IL7-0.7092515236252220.003703227247480960.030995251265146
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.424 0.03673

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.097272986
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)
Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0700554 0.68608
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.971 1.013 0.97 0.891 0.919
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found

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Contacts

Dr. Chen Li
Address: Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Australia, VIC 3800
Email: Chen Li at Monash

© 2017 HIVed Database, Purcell Lab, Department of Biochemistry and Molecular Biology, Monash University. All rights reserved.
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