Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001449
UniProt IDQ8TEX9
Primary gene name(s)IPO4
Synonym gene name(s)IMP4B, RANBP4
Protein nameImportin-4
Protein functionFunctions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals, NLS in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex, NPC through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus, By similarity. Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. {ECO:0000250, ECO:0000269|PubMed:11823430}.
Subcellular locationCytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8TEX9
Gene Ontology
(Biological Process)
Complete annatation
DNA replication-dependent nucleosome assembly [GO:0006335];
DNA replication-independent nucleosome assembly [GO:0006336];
NLS-bearing protein import into nucleus [GO:0006607];
protein import into nucleus, docking [GO:0000059];
protein import into nucleus, translocation [GO:0000060];
protein transport [GO:0015031];
ribosomal protein import into nucleus [GO:0006610]
Gene Ontology
(Molecular Function)
Complete annatation
nuclear localization sequence binding [GO:0008139];
protein transporter activity [GO:0008565]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
membrane [GO:0016020];
nuclear chromatin [GO:0000790];
nuclear membrane [GO:0031965];
nuclear periphery [GO:0034399];
protein complex [GO:0043234]
Protein-protein interaction122828
Phylogenetic treeQ8TEX9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.178332695883716.78954188992886e-064.36383690255897e-05
AZA vs. DISU-0.2551195622445570.3182524090158960.870080690516335
AZA vs. IL70.6537753138347550.002168460492083750.139680363235805
AZA vs. SAHA-0.2561723854081480.3006264300720650.672749822878015
DISU vs. CD3-2.445732748727265.69993645838096e-075.84495524990712e-06
DISU vs. IL70.8999727477797850.0004295961158256030.0157188384613373
DISU vs. SAHA0.0006577062393145560.9982149855646170.999572425883731
DMSO vs. AZA0.1110622949958060.5881788163926481
DMSO vs. CD3-2.082072593835248.27100521294533e-064.84081948457736e-05
DMSO vs. DISU0.3633644544137320.1405662602182860.633424573410949
DMSO vs. IL70.5507252706996290.002565584081391510.0991696923768643
DMSO vs. SAHA-0.3722326503795360.1190060642850430.410515082362068
HIV vs. Mock in Activation0.104944739041250.9140236703506930.999983755607037
HIV vs. Mock in Latency-0.1452847834749120.3941873244357930.999834320637052
IL7 vs. CD3-1.517259378891580.000970471223666780.00413101179167732
SAHA vs. CD3-2.458368294290381.48185389181599e-071.48087123008401e-06
SAHA vs. IL7-0.9127902279386320.0002259380838041110.00367795969250883
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.485337197
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.100485 0.631594
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.953 0.92 0.818 0.735 0.864
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
integrase interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found