Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001448
UniProt IDO94829
Primary gene name(s)IPO13
Synonym gene name(s)KIAA0724, RANBP13
Protein nameImportin-13
Protein functionFunctions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals, NLS in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex, NPC through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus, By similarity. Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13. {ECO:0000250, ECO:0000269|PubMed:11447110, ECO:0000269|PubMed:15143176}.
Subcellular locationCytoplasm. Nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O94829
Gene Ontology
(Biological Process)
Complete annatation
protein import into nucleus [GO:0006606]
Gene Ontology
(Molecular Function)
Complete annatation
nuclear localization sequence binding [GO:0008139];
protein transporter activity [GO:0008565]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear membrane [GO:0031965]
Protein-protein interaction115025
Phylogenetic treeO94829
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3266580131510530.3198968720823920.439515556611286
AZA vs. DISU0.2266756421394570.372708355613420.898161104681239
AZA vs. IL7-0.03823414455955390.8440455608436480.999311006273513
AZA vs. SAHA-0.1220626740160940.6192839056185720.876662301925958
DISU vs. CD3-0.1124504065737130.7567474215668980.831082392046461
DISU vs. IL7-0.2746658421954270.2780645017799610.665759604048733
DISU vs. SAHA-0.3460283223522710.2373068910903320.615780329824793
DMSO vs. AZA0.02792980650019050.8695104457198141
DMSO vs. CD3-0.311606012679610.3311123545036740.44501807659897
DMSO vs. DISU-0.2010419064528820.4122348859256910.871912944687742
DMSO vs. IL7-0.05851308889935160.7475901611349720.950566607561045
DMSO vs. SAHA-0.1545877046266830.5148154690468780.821285617406085
HIV vs. Mock in Activation0.08320267821708090.8937648414686980.999983755607037
HIV vs. Mock in Latency0.00290413378247610.9861642011502690.999834320637052
IL7 vs. CD3-0.3596010861696380.2644728481153340.398082608312605
SAHA vs. CD3-0.4715365610930690.1834860663977910.282387154883039
SAHA vs. IL7-0.08588455086271760.7260239620238970.874826954720081
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0220345 0.93127
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2X19 X-ray 2.8Å B=1-963.
2XWU X-ray 2.8Å B=1-963.
3ZJY X-ray 3.6Å B/E/G=1-963.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found