Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001443
UniProt IDQ68E01
Primary gene name(s)INTS3
Synonym gene name(s)C1orf193, C1orf60
Protein nameIntegrator complex subunit 3
Protein functionComponent of the Integrator complex. The Integrator complex is involved in the small nuclear RNAs, snRNA U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain, CTD of RNA polymerase II largest subunit, POLR2A and is recruited to the U1 and U2 snRNAs genes.; FUNCTION: Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. The SOSS complex is required for efficient homologous recombination-dependent repair of double-strand breaks, DSBs and ATM-dependent signaling pathways. In the SOSS complex, it is required for the assembly of the complex and for stabilization of the complex at DNA damage sites.
Subcellular locationNucleus {ECO:0000269|PubMed:19605351, ECO:0000269|PubMed:19683501}. Note=Localizes to nuclear foci following DNA damage.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q68E01
Gene Ontology
(Biological Process)
Complete annatation
cellular response to DNA damage stimulus [GO:0006974];
DNA repair [GO:0006281];
mitotic cell cycle checkpoint [GO:0007093];
response to ionizing radiation [GO:0010212];
snRNA processing [GO:0016180];
snRNA transcription from RNA polymerase II promoter [GO:0042795]
Gene Ontology
(Molecular Function)
Complete annatation
unknown
Gene Ontology
(Cellular Component)
Complete annatation
integrator complex [GO:0032039];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
SOSS complex [GO:0070876]
Protein-protein interaction122401
Phylogenetic treeQ68E01
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.01708423711599270.9584491426215240.972319140983483
AZA vs. DISU-0.08865809403979360.7261605430954330.974429666417572
AZA vs. IL70.03659231270766250.8492068704855250.999311006273513
AZA vs. SAHA-0.1667651867622980.4982692869959680.820211358158055
DISU vs. CD3-0.0841270710143760.8172056682964470.875493015001592
DISU vs. IL70.1155279796154260.6469622689562080.897815155726679
DISU vs. SAHA-0.07558337404962540.7981854344268580.946829953483023
DMSO vs. AZA0.07386197275726190.6598048176893611
DMSO vs. CD30.08077598646648550.8008879792082250.859454311935535
DMSO vs. DISU0.1611108123349190.5093139256041360.911454798581442
DMSO vs. IL7-0.03019315364581130.86677947571070.970618094663316
DMSO vs. SAHA-0.2463757265056190.3007909227263590.653672674319965
HIV vs. Mock in Activation0.1707049848632370.7837384250794980.999983755607037
HIV vs. Mock in Latency0.06331383709473640.7010178414246860.999834320637052
IL7 vs. CD30.05916029605561470.8539184169618910.906113580126464
SAHA vs. CD3-0.1733218499057880.624257317450780.717890784543464
SAHA vs. IL7-0.2056810008210940.4015537447801750.648491659397511
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0668085 0.728841
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.004 1.185 1.087 1.081 1.293
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4OWT X-ray 2.0Å A=35-499.
4OWW X-ray 2.3Å A=1-501.
4OWX X-ray 2.3Å A=1-501.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found
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