Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001440
UniProt IDO15503
Primary gene name(s)INSIG1
Synonym gene name(s)unknown
Protein nameInsulin-induced gene 1 protein
Protein functionMediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins, SREBPs. Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation, ERAD of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. {ECO:0000269|PubMed:12202038, ECO:0000269|PubMed:12535518, ECO:0000269|PubMed:16168377, ECO:0000269|PubMed:16399501, ECO:0000269|PubMed:16606821}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:12202038};
Multi-pass membrane protein {ECO:0000269|PubMed:12202038}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O15503
Gene Ontology
(Biological Process)
Complete annatation
cell proliferation [GO:0008283];
cholesterol biosynthetic process [GO:0006695];
cranial suture morphogenesis [GO:0060363];
inner ear morphogenesis [GO:0042472];
metabolic process [GO:0008152];
middle ear morphogenesis [GO:0042474];
negative regulation of cargo loading into COPII-coated vesicle [GO:1901303];
negative regulation of fat cell differentiation [GO:0045599];
negative regulation of fatty acid biosynthetic process [GO:0045717];
negative regulation of steroid biosynthetic process [GO:0010894];
palate development [GO:0060021];
SREBP signaling pathway [GO:0032933];
triglyceride metabolic process [GO:0006641]
Gene Ontology
(Molecular Function)
Complete annatation
unknown
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
SREBP-SCAP-Insig complex [GO:0032937]
Protein-protein interaction109850
Phylogenetic treeO15503
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.719284746828670.1191221803243780.202459348426819
AZA vs. DISU0.47601800115540.06084382969618660.549526596069693
AZA vs. IL7-0.1914954454302170.5319629302915150.999311006273513
AZA vs. SAHA-0.3338503679741940.1736968710343280.531081008784093
DISU vs. CD3-0.2558584092915340.5556571027024330.674009917184913
DISU vs. IL7-0.676773067189510.009421409030017870.113259761664688
DISU vs. SAHA-0.8086611958201870.00583233952492790.071943307726732
DMSO vs. AZA-0.08116078242177740.7525730770482771
DMSO vs. CD3-0.8117824247831280.07618720417401660.136737707399513
DMSO vs. DISU-0.5591701688016480.02251782165493080.307233904729923
DMSO vs. IL7-0.1030378129267630.7285310353806830.94524644007606
DMSO vs. SAHA-0.2593059113333640.2738263376116310.622715757426279
HIV vs. Mock in Activation-0.2522895125108340.7871036759870710.999983755607037
HIV vs. Mock in Latency0.2967871759370130.1367001526048170.999834320637052
IL7 vs. CD3-0.9031051962733420.05803231911758610.124126741316784
SAHA vs. CD3-1.077481581667470.01202264033465790.0299671170762976
SAHA vs. IL7-0.1459342963972210.5535036163620850.765666104954315
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0277353 0.888179
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4J81 X-ray 1.7Å C/D=273-277.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vif upregulates 23333304

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found
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