Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001433
UniProt IDQ9UNL4
Primary gene name(s)ING4
Synonym gene name(s)unknown
Protein nameInhibitor of growth protein 4
Protein functionComponent of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's, HIF activity by interacting with HIF prolyl hydroxylase 2, EGLN1. Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11, By similarity. {ECO:0000250|UniProtKB:Q8C0D7, ECO:0000269|PubMed:12750254, ECO:0000269|PubMed:15029197, ECO:0000269|PubMed:15251430, ECO:0000269|PubMed:15528276, ECO:0000269|PubMed:15897452, ECO:0000269|PubMed:16387653}.
Subcellular locationNucleus {ECO:0000269|PubMed:15029197, ECO:0000269|PubMed:16973615}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UNL4
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
cell cycle arrest [GO:0007050];
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator [GO:0006978];
DNA replication [GO:0006260];
histone H3 acetylation [GO:0043966];
histone H4-K12 acetylation [GO:0043983];
histone H4-K5 acetylation [GO:0043981];
histone H4-K8 acetylation [GO:0043982];
negative regulation of cell proliferation [GO:0008285];
negative regulation of growth [GO:0045926];
negative regulation of transcription, DNA-templated [GO:0045892];
positive regulation of apoptotic process [GO:0043065];
protein acetylation [GO:0006473]
Gene Ontology
(Molecular Function)
Complete annatation
methylated histone binding [GO:0035064];
transcription coactivator activity [GO:0003713];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
histone acetyltransferase complex [GO:0000123];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction119331
Phylogenetic treeQ9UNL4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.5951142742122.1322416726699e-061.53619247086428e-05
AZA vs. DISU-0.02089763317500570.9343490512904050.995042856396371
AZA vs. IL7-0.57967488684630.002878910705199630.159536106836302
AZA vs. SAHA-0.09224583254970020.7063777218535080.91681931791089
DISU vs. CD31.560484487648112.72102024133636e-050.000179503978634154
DISU vs. IL7-0.5679622813630290.02518565683226440.201846771263363
DISU vs. SAHA-0.06930090623510340.8124101684309230.951385325391594
DMSO vs. AZA-0.003809493075284460.9820145274017851
DMSO vs. CD31.576522101169521.59184451686833e-061.09603005697539e-05
DMSO vs. DISU0.01425906682437640.9535191577576280.993828264951078
DMSO vs. IL7-0.5682551911227080.001792276699830620.0820330629509829
DMSO vs. SAHA-0.09380623473850970.6917573000130780.905576215888824
HIV vs. Mock in Activation-0.1950962016847940.7551936222848940.999983755607037
HIV vs. Mock in Latency0.06374810864408940.7008618554853180.999834320637052
IL7 vs. CD31.022880699052240.001700052314100750.00669307293617182
SAHA vs. CD31.478609380482014.34603353298701e-050.00023029087673036
SAHA vs. IL70.4839741946557910.04842624183087070.179967173866455
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.450509 0.00261319
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.051 0.936 0.912 0.974 1.022
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2K1J NMR - A=188-249.
2M1R NMR - A=188-249.
2PNX X-ray 1.8Å A/C=194-246.
2VNF X-ray 1.7Å A/C=188-246.
4AFL X-ray 2.2Å A/B/C/D/E/F=2-105.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found