Protein Information (annotations from UniProt)
| Database ID | HIV0001432 |
| UniProt ID | Q9NQS7 |
| Primary gene name(s) | INCENP |
| Synonym gene name(s) | unknown |
| Protein name | Inner centromere protein |
| Protein function | Component of the chromosomal passenger complex, CPC, a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus asssociated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules, PubMed:15316025, PubMed:12925766, PubMed:27332895. The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin, By similarity. Activates AURKB and AURKC, PubMed:27332895. Required for localization of CBX5 to mitotic centromeres, PubMed:21346195. Controls the kinetochore localization of BUB1, PubMed:16760428. {ECO:0000250|UniProtKB:P53352, ECO:0000269|PubMed:12925766, ECO:0000269|PubMed:15316025, ECO:0000269|PubMed:16760428, ECO:0000269|PubMed:21346195, ECO:0000269|PubMed:27332895}. |
| Subcellular location | Nucleus {ECO:0000269|PubMed:11453556}. Chromosome, centromere {ECO:0000269|PubMed:11453556, ECO:0000269|PubMed:12925766, ECO:0000269|PubMed:15316025, ECO:0000269|PubMed:16760428}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:11453556, ECO:0000269|PubMed:15316025}. Midbody {ECO:0000269|PubMed:15316025}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:14610074}. Note=Colocalized at synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocenters. Colocalizes with AURKB at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone, By similarity. Colocalizes with AURKB at mitotic chromosomes, PubMed:11453556. Localizes to inner kinetochore, PubMed:16760428. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis, PubMed:15316025. Cocalizes to the equatorial cell cortex at anaphase, PubMed:11453556. {ECO:0000250|UniProtKB:Q9WU62, ECO:0000269|PubMed:11453556, ECO:0000269|PubMed:15316025, ECO:0000269|PubMed:16760428}. |
| ECO code | Click here for more information. |
| Amino acid sequence FASTA format: Q9NQS7 |
|
| Gene Ontology (Biological Process) Complete annatation | chromosome segregation [GO:0007059]; cytokinesis [GO:0000910]; mitotic nuclear division [GO:0007067]; protein sumoylation [GO:0016925]; sister chromatid cohesion [GO:0007062] |
| Gene Ontology (Molecular Function) Complete annatation | unknown |
| Gene Ontology (Cellular Component) Complete annatation | central element [GO:0000801]; chromocenter [GO:0010369]; chromosome, centromeric region [GO:0000775]; condensed chromosome kinetochore [GO:0000777]; cytosol [GO:0005829]; kinetochore [GO:0000776]; lateral element [GO:0000800]; microtubule [GO:0005874]; midbody [GO:0030496]; nucleoplasm [GO:0005654]; pericentric heterochromatin [GO:0005721]; protein complex [GO:0043234]; spindle [GO:0005819] |
| Protein-protein interaction | 109831 |
| Phylogenetic tree | Q9NQS7 |
| HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
| Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
| Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Gene Expression Profile top
Up-regulated; 
Down-regulated
For brief introduction to each study, please go to the help page.
Gene expression during HIV latency
| (1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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| Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
| AZA vs. CD3 | -0.144494626523049 | 0.660746503810794 | 0.754335594867261 |
| AZA vs. DISU | 0.660243115117844 | 0.0667646550276489 | 0.565919074643146 |
| AZA vs. IL7 | -0.0419956720606325 | 0.830252859107314 | 0.999311006273513 |
| AZA vs. SAHA | 0.0438763611174041 | 0.858977982996942 | 0.967133804138028 |
| DISU vs. CD3 | 0.791932194422319 | 0.0395500347223764 | 0.089381892564483 |
| DISU vs. IL7 | -0.711603716330148 | 0.0523033806420176 | 0.301981410638995 |
| DISU vs. SAHA | -0.614177097646029 | 0.106117216160463 | 0.41778802632036 |
| DMSO vs. AZA | 0.0616568695894017 | 0.719205480915852 | 1 |
| DMSO vs. CD3 | 0.194572925097604 | 0.54476475706737 | 0.648983506525069 |
| DMSO vs. DISU | -0.600611633675578 | 0.098458869542248 | 0.56675903896168 |
| DMSO vs. IL7 | -0.0963239204691611 | 0.598897401629113 | 0.911970258876874 |
| DMSO vs. SAHA | -0.0236876305939416 | 0.920953663360971 | 0.981868467778239 |
| HIV vs. Mock in Activation | 0.493128757310049 | 0.431087319981054 | 0.999983755607037 |
| HIV vs. Mock in Latency | 0.111883292519644 | 0.50852186218911 | 0.999834320637052 |
| IL7 vs. CD3 | 0.10851562212086 | 0.736747611901095 | 0.823080103334213 |
| SAHA vs. CD3 | 0.164643867665031 | 0.643977679091194 | 0.734706913763668 |
| SAHA vs. IL7 | 0.083177603891698 | 0.736780042082848 | 0.88046792090985 |
| (2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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| Log2 Fold Change | P Value | ||
| unknown | unknown | ||
Gene expression during HIV infection and replication
| (1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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| Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
| Infected vs. Mock | unknown | unknown | unknown | ||
| Infected vs. Bystander | unknown | unknown | unknown | ||
| (2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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| Log2 Fold Change | unknown | ||||
| (3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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| Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
| unknown | unknown | unknown | unknown | unknown | unknown |
| (4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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| Up-regulated (True) | FALSE | ||||
| (5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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| Test Status | Log2 Fold Change | P Value | |||
| OK | 0.0466286 | 0.81168 | |||
| (6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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| Log2 Fold Change | P Value | ||||
| unknown | unknown | ||||
Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV
| (1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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| 6 h | 24 h | 48 h | 72 h | RTi | ||
| 1.14 | 1.345 | 1.739 | 1.552 | 1.094 | ||
| (2). Navare et al., Virology, 2012 SUP-T1 cell line |
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| FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
| unknown | unknown | unknown | unknown | unknown | unknown | unknown |
| (3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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| Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
| unknown | unknown | unknown | unknown | |||
Protein Overview top
Drug-protein Interaction (annotations from DrugBank) top
| Drugbank ID | Drug Name | Drug Status | Pharmacological Action | Drug Action |
|---|---|---|---|---|
| DB07340 | N~6~-cyclohexyl-N~2~-(4-morpholin-4-ylphenyl)-9H-purine-2,6-diamine | experimental | unknown | unknown |
Protein Secondary Structure (annotations from PDB) top
HIV-1 Interaction (annotations from NCBI HIV-1 Interaction Database) top
Metabolic/Signalling Pathway (annotations from KEGG database) top
| Pathway Accession Number | Description | not found |
|---|