Database ID | HIV0001432 |
UniProt ID | Q9NQS7 |
Primary gene name(s) | INCENP |
Synonym gene name(s) | unknown |
Protein name | Inner centromere protein |
Protein function | Component of the chromosomal passenger complex, CPC, a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus asssociated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules, PubMed:15316025, PubMed:12925766, PubMed:27332895. The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin, By similarity. Activates AURKB and AURKC, PubMed:27332895. Required for localization of CBX5 to mitotic centromeres, PubMed:21346195. Controls the kinetochore localization of BUB1, PubMed:16760428. {ECO:0000250|UniProtKB:P53352, ECO:0000269|PubMed:12925766, ECO:0000269|PubMed:15316025, ECO:0000269|PubMed:16760428, ECO:0000269|PubMed:21346195, ECO:0000269|PubMed:27332895}. |
Subcellular location | Nucleus {ECO:0000269|PubMed:11453556}. Chromosome, centromere {ECO:0000269|PubMed:11453556, ECO:0000269|PubMed:12925766, ECO:0000269|PubMed:15316025, ECO:0000269|PubMed:16760428}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:11453556, ECO:0000269|PubMed:15316025}. Midbody {ECO:0000269|PubMed:15316025}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:14610074}. Note=Colocalized at synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocenters. Colocalizes with AURKB at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone, By similarity. Colocalizes with AURKB at mitotic chromosomes, PubMed:11453556. Localizes to inner kinetochore, PubMed:16760428. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis, PubMed:15316025. Cocalizes to the equatorial cell cortex at anaphase, PubMed:11453556. {ECO:0000250|UniProtKB:Q9WU62, ECO:0000269|PubMed:11453556, ECO:0000269|PubMed:15316025, ECO:0000269|PubMed:16760428}. |
ECO code | Click here for more information. |
Amino acid sequence FASTA format: Q9NQS7 |
|
Gene Ontology (Biological Process) Complete annatation | chromosome segregation [GO:0007059]; cytokinesis [GO:0000910]; mitotic nuclear division [GO:0007067]; protein sumoylation [GO:0016925]; sister chromatid cohesion [GO:0007062] |
Gene Ontology (Molecular Function) Complete annatation | unknown |
Gene Ontology (Cellular Component) Complete annatation | central element [GO:0000801]; chromocenter [GO:0010369]; chromosome, centromeric region [GO:0000775]; condensed chromosome kinetochore [GO:0000777]; cytosol [GO:0005829]; kinetochore [GO:0000776]; lateral element [GO:0000800]; microtubule [GO:0005874]; midbody [GO:0030496]; nucleoplasm [GO:0005654]; pericentric heterochromatin [GO:0005721]; protein complex [GO:0043234]; spindle [GO:0005819] |
Protein-protein interaction | 109831 |
Phylogenetic tree | Q9NQS7 |
HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Up-regulated;
Down-regulated
For brief introduction to each study, please go to the help page.
(1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
|||
Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
AZA vs. CD3 | -0.144494626523049 | 0.660746503810794 | 0.754335594867261 |
AZA vs. DISU | 0.660243115117844 | 0.0667646550276489 | 0.565919074643146 |
AZA vs. IL7 | -0.0419956720606325 | 0.830252859107314 | 0.999311006273513 |
AZA vs. SAHA | 0.0438763611174041 | 0.858977982996942 | 0.967133804138028 |
DISU vs. CD3 | 0.791932194422319 | 0.0395500347223764 | 0.089381892564483 |
DISU vs. IL7 | -0.711603716330148 | 0.0523033806420176 | 0.301981410638995 |
DISU vs. SAHA | -0.614177097646029 | 0.106117216160463 | 0.41778802632036 |
DMSO vs. AZA | 0.0616568695894017 | 0.719205480915852 | 1 |
DMSO vs. CD3 | 0.194572925097604 | 0.54476475706737 | 0.648983506525069 |
DMSO vs. DISU | -0.600611633675578 | 0.098458869542248 | 0.56675903896168 |
DMSO vs. IL7 | -0.0963239204691611 | 0.598897401629113 | 0.911970258876874 |
DMSO vs. SAHA | -0.0236876305939416 | 0.920953663360971 | 0.981868467778239 |
HIV vs. Mock in Activation | 0.493128757310049 | 0.431087319981054 | 0.999983755607037 |
HIV vs. Mock in Latency | 0.111883292519644 | 0.50852186218911 | 0.999834320637052 |
IL7 vs. CD3 | 0.10851562212086 | 0.736747611901095 | 0.823080103334213 |
SAHA vs. CD3 | 0.164643867665031 | 0.643977679091194 | 0.734706913763668 |
SAHA vs. IL7 | 0.083177603891698 | 0.736780042082848 | 0.88046792090985 |
(2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
|||
Log2 Fold Change | P Value | ||
unknown | unknown |
(1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
|||||
Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
Infected vs. Mock | unknown | unknown | unknown | ||
Infected vs. Bystander | unknown | unknown | unknown | ||
(2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
|||||
Log2 Fold Change | unknown | ||||
(3). Li et al., J. Immunol., 2013 Lymphatic tissue |
|||||
Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
unknown | unknown | unknown | unknown | unknown | unknown |
(4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
|||||
Up-regulated (True) | FALSE | ||||
(5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
|||||
Test Status | Log2 Fold Change | P Value | |||
OK | 0.0466286 | 0.81168 | |||
(6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
|||||
Log2 Fold Change | P Value | ||||
unknown | unknown |
(1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
||||||
6 h | 24 h | 48 h | 72 h | RTi | ||
1.14 | 1.345 | 1.739 | 1.552 | 1.094 | ||
(2). Navare et al., Virology, 2012 SUP-T1 cell line |
||||||
FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
unknown | unknown | unknown | unknown | unknown | unknown | unknown |
(3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
||||||
Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
unknown | unknown | unknown | unknown |
Drugbank ID | Drug Name | Drug Status | Pharmacological Action | Drug Action |
---|---|---|---|---|
DB07340 | N~6~-cyclohexyl-N~2~-(4-morpholin-4-ylphenyl)-9H-purine-2,6-diamine | experimental | unknown | unknown |
Pathway Accession Number | Description | not found |
---|