Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001432
UniProt IDQ9NQS7
Primary gene name(s)INCENP
Synonym gene name(s)unknown
Protein nameInner centromere protein
Protein functionComponent of the chromosomal passenger complex, CPC, a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus asssociated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules, PubMed:15316025, PubMed:12925766, PubMed:27332895. The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin, By similarity. Activates AURKB and AURKC, PubMed:27332895. Required for localization of CBX5 to mitotic centromeres, PubMed:21346195. Controls the kinetochore localization of BUB1, PubMed:16760428. {ECO:0000250|UniProtKB:P53352, ECO:0000269|PubMed:12925766, ECO:0000269|PubMed:15316025, ECO:0000269|PubMed:16760428, ECO:0000269|PubMed:21346195, ECO:0000269|PubMed:27332895}.
Subcellular locationNucleus {ECO:0000269|PubMed:11453556}. Chromosome, centromere {ECO:0000269|PubMed:11453556, ECO:0000269|PubMed:12925766, ECO:0000269|PubMed:15316025, ECO:0000269|PubMed:16760428}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:11453556, ECO:0000269|PubMed:15316025}. Midbody {ECO:0000269|PubMed:15316025}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:14610074}. Note=Colocalized at synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocenters. Colocalizes with AURKB at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone, By similarity. Colocalizes with AURKB at mitotic chromosomes, PubMed:11453556. Localizes to inner kinetochore, PubMed:16760428. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis, PubMed:15316025. Cocalizes to the equatorial cell cortex at anaphase, PubMed:11453556. {ECO:0000250|UniProtKB:Q9WU62, ECO:0000269|PubMed:11453556, ECO:0000269|PubMed:15316025, ECO:0000269|PubMed:16760428}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NQS7
Gene Ontology
(Biological Process)
Complete annatation
chromosome segregation [GO:0007059];
cytokinesis [GO:0000910];
mitotic nuclear division [GO:0007067];
protein sumoylation [GO:0016925];
sister chromatid cohesion [GO:0007062]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
central element [GO:0000801];
chromocenter [GO:0010369];
chromosome, centromeric region [GO:0000775];
condensed chromosome kinetochore [GO:0000777];
cytosol [GO:0005829];
kinetochore [GO:0000776];
lateral element [GO:0000800];
microtubule [GO:0005874];
midbody [GO:0030496];
nucleoplasm [GO:0005654];
pericentric heterochromatin [GO:0005721];
protein complex [GO:0043234];
spindle [GO:0005819]
Protein-protein interaction109831
Phylogenetic treeQ9NQS7
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1444946265230490.6607465038107940.754335594867261
AZA vs. DISU0.6602431151178440.06676465502764890.565919074643146
AZA vs. IL7-0.04199567206063250.8302528591073140.999311006273513
AZA vs. SAHA0.04387636111740410.8589779829969420.967133804138028
DISU vs. CD30.7919321944223190.03955003472237640.089381892564483
DISU vs. IL7-0.7116037163301480.05230338064201760.301981410638995
DISU vs. SAHA-0.6141770976460290.1061172161604630.41778802632036
DMSO vs. AZA0.06165686958940170.7192054809158521
DMSO vs. CD30.1945729250976040.544764757067370.648983506525069
DMSO vs. DISU-0.6006116336755780.0984588695422480.56675903896168
DMSO vs. IL7-0.09632392046916110.5988974016291130.911970258876874
DMSO vs. SAHA-0.02368763059394160.9209536633609710.981868467778239
HIV vs. Mock in Activation0.4931287573100490.4310873199810540.999983755607037
HIV vs. Mock in Latency0.1118832925196440.508521862189110.999834320637052
IL7 vs. CD30.108515622120860.7367476119010950.823080103334213
SAHA vs. CD30.1646438676650310.6439776790911940.734706913763668
SAHA vs. IL70.0831776038916980.7367800420828480.88046792090985
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0466286 0.81168
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.14 1.345 1.739 1.552 1.094
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB07340 N~6~-cyclohexyl-N~2~-(4-morpholin-4-ylphenyl)-9H-purine-2,6-diamine experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2QFA X-ray 1.4Å C=1-47.
4AF3 X-ray 2.7Å D=835-903.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr downregulates 21875947
Vpr relocalizes 21875947

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found