Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001428
UniProt IDQ13418
Primary gene name(s)ILK
Synonym gene name(s)ILK1, ILK2
Protein nameIntegrin-linked protein kinase
Protein functionReceptor-proximal protein kinase regulating integrin-mediated signal transduction, PubMed:8538749, PubMed:9736715. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B, PubMed:8538749, PubMed:9736715. {ECO:0000269|PubMed:8538749, ECO:0000269|PubMed:9736715, ECO:0000303|PubMed:10712922}.
Subcellular locationCell junction, focal adhesion {ECO:0000269|PubMed:11402068, ECO:0000269|PubMed:12167643}. Cell membrane;
Peripheral membrane protein;
Cytoplasmic side {ECO:0000269|PubMed:11402068}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:O55222}. Cytoplasm, myofibril, sarcomere {ECO:0000269|PubMed:11402068}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13418
Gene Ontology
(Biological Process)
Complete annatation
branching involved in ureteric bud morphogenesis [GO:0001658];
cell aging [GO:0007569];
cell cycle arrest [GO:0007050];
cell junction assembly [GO:0034329];
cell-matrix adhesion [GO:0007160];
cell proliferation [GO:0008283];
establishment or maintenance of epithelial cell apical/basal polarity [GO:0045197];
extracellular fibril organization [GO:0043206];
fibroblast migration [GO:0010761];
integrin-mediated signaling pathway [GO:0007229];
myelin assembly [GO:0032288];
myelination in peripheral nervous system [GO:0022011];
negative regulation of cardiac muscle cell apoptotic process [GO:0010667];
negative regulation of neural precursor cell proliferation [GO:2000178];
negative regulation of neuron apoptotic process [GO:0043524];
negative regulation of protein kinase activity [GO:0006469];
negative regulation of smooth muscle cell migration [GO:0014912];
negative regulation of smooth muscle cell proliferation [GO:0048662];
nerve development [GO:0021675];
neuron projection morphogenesis [GO:0048812];
outflow tract morphogenesis [GO:0003151];
peptidyl-serine phosphorylation [GO:0018105];
platelet aggregation [GO:0070527];
positive regulation of axon extension [GO:0045773];
positive regulation of BMP signaling pathway [GO:0030513];
positive regulation of canonical Wnt signaling pathway [GO:0090263];
positive regulation of cell-matrix adhesion [GO:0001954];
positive regulation of cell migration [GO:0030335];
positive regulation of cell proliferation [GO:0008284];
positive regulation of dendrite morphogenesis [GO:0050775];
positive regulation of MAP kinase activity [GO:0043406];
positive regulation of myoblast differentiation [GO:0045663];
positive regulation of osteoblast differentiation [GO:0045669];
positive regulation of phosphorylation [GO:0042327];
positive regulation of protein kinase B signaling [GO:0051897];
positive regulation of transcription, DNA-templated [GO:0045893];
protein heterooligomerization [GO:0051291];
protein kinase B signaling [GO:0043491];
protein phosphorylation [GO:0006468];
regulation of actin cytoskeleton organization [GO:0032956];
substrate adhesion-dependent cell spreading [GO:0034446]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
protein kinase binding [GO:0019901];
protein serine/threonine kinase activity [GO:0004674];
signal transducer activity [GO:0004871]
Gene Ontology
(Cellular Component)
Complete annatation
cell-cell junction [GO:0005911];
cell junction [GO:0030054];
costamere [GO:0043034];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
dendritic shaft [GO:0043198];
extracellular matrix [GO:0031012];
focal adhesion [GO:0005925];
lamellipodium [GO:0030027];
membrane [GO:0016020];
neuronal cell body [GO:0043025];
nucleoplasm [GO:0005654];
plasma membrane [GO:0005886];
protein complex [GO:0043234];
sarcomere [GO:0030017];
stress fiber [GO:0001725];
terminal bouton [GO:0043195]
Protein-protein interaction109824
Phylogenetic treeQ13418
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.8247389074067110.0137087326555720.0340616581906993
AZA vs. DISU0.247373966037270.3286690497824010.878012646527526
AZA vs. IL7-0.1034544368047950.5918222509387290.999311006273513
AZA vs. SAHA0.3065823304927720.2101925288737770.578816620431736
DISU vs. CD31.059516568001590.004413063848257350.0146203350436552
DISU vs. IL7-0.3606471898459510.1533151464087620.508520535118172
DISU vs. SAHA0.06200311734053350.8315477383204820.956295762245554
DMSO vs. AZA0.04571617431003680.7857950521425161
DMSO vs. CD30.8591736121283080.008488428762375320.0216632524657266
DMSO vs. DISU-0.2035256252930880.4046454557925270.868224804711215
DMSO vs. IL7-0.1419222942093230.4315214187749530.85854367005839
DMSO vs. SAHA0.2556403784391930.279608989115680.628109565503414
HIV vs. Mock in Activation0.09926526178516730.8844373404659120.999983755607037
HIV vs. Mock in Latency0.1238387484090120.4544565640537290.999834320637052
IL7 vs. CD30.7269038308840030.02582965829515670.0642883816847584
SAHA vs. CD31.108260895493270.002455885484805690.00771849723796073
SAHA vs. IL70.4081279733685340.09565676933329040.280084719152395
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.098025 0.677031
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.037 1.131 1.274 1.413 1.201
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
201234_at 1.39 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2KBX NMR - A=1-171.
3F6Q X-ray 1.6Å A=1-170.
3IXE X-ray 1.9Å A=1-174.
3KMU X-ray 1.8Å A=183-452.
3KMW X-ray 2.0Å A=183-452.
3REP X-ray 1.8Å A=183-452.
4HI8 X-ray 1.2Å A=1-174.
4HI9 X-ray 1.2Å A=1-174.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03320 PPAR signaling pathway - Homo sapiens (human)
hsa04360 Axon guidance - Homo sapiens (human)
hsa04510 Focal adhesion - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05213 Endometrial cancer - Homo sapiens (human)
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