Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001384
UniProt IDO43719
Primary gene name(s)HTATSF1
Synonym gene name(s)unknown
Protein nameHIV Tat-specific factor 1
Protein functionFunctions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus. {ECO:0000269|PubMed:10393184, ECO:0000269|PubMed:10454543, ECO:0000269|PubMed:10913173, ECO:0000269|PubMed:11420046, ECO:0000269|PubMed:11780068, ECO:0000269|PubMed:15905670, ECO:0000269|PubMed:8849451, ECO:0000269|PubMed:9765201}.
Subcellular locationNucleus {ECO:0000269|PubMed:15485897, ECO:0000269|PubMed:8849451}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O43719
Gene Ontology
(Biological Process)
Complete annatation
mRNA splicing, via spliceosome [GO:0000398];
regulation of DNA-templated transcription, elongation [GO:0032784];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
transcription, DNA-templated [GO:0006351];
viral genome replication [GO:0019079]
Gene Ontology
(Molecular Function)
Complete annatation
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
RNA binding [GO:0003723]
Gene Ontology
(Cellular Component)
Complete annatation
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
U2 snRNP [GO:0005686];
U2-type spliceosomal complex [GO:0005684]
Protein-protein interaction118149
Phylogenetic treeO43719
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.874990443436090.008065745336023890.021743322072
AZA vs. DISU-0.4407234081988950.08147045058362080.600953095263905
AZA vs. IL70.3657884754742350.05665611244854830.690210880962922
AZA vs. SAHA-0.1177634091642620.62980172529430.882826402174051
DISU vs. CD30.4230177882182750.2453947827076830.371729570348268
DISU vs. IL70.7966336309405030.00164629397377380.0376591704536265
DISU vs. SAHA0.3247560244398470.2655878777375020.643827393498775
DMSO vs. AZA-0.0473082141980950.7774436780464191
DMSO vs. CD30.8220726590399440.01072944922823670.0264635154917135
DMSO vs. DISU0.3932408292954750.1069213621521940.581051936518753
DMSO vs. IL70.4194809774940570.01947565449051040.288729693514844
DMSO vs. SAHA-0.07786330339870980.746695737367630.926362484184026
HIV vs. Mock in Activation-0.004282060494297680.9945026453837620.999983755607037
HIV vs. Mock in Latency0.01600672233468050.9608004503106530.999834320637052
IL7 vs. CD31.246961920621440.0001305760708925070.000736867509008897
SAHA vs. CD30.7336994928088560.04108221832142370.0842210624546123
SAHA vs. IL7-0.4858915080911870.04718090038601950.176800886238064
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.13146 0.384526
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.002 1.348 1.52 1.403 2.03
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2DIT NMR - A=256-354.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat associates with 19966273
Tat stimulated by 8849451
HIV-1 virus replication enhanced by expression of human gene 18187620
Nef upregulates 11420046

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found