Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001381
UniProt IDP10809
Primary gene name(s)HSPD1
Synonym gene name(s)HSP60
Protein name60 kDa heat shock protein, mitochondrial
Protein functionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Subcellular locationMitochondrion matrix.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P10809
Gene Ontology
(Biological Process)
Complete annatation
'de novo' protein folding [GO:0006458];
activation of cysteine-type endopeptidase activity involved in apoptotic process [GO:0006919];
B cell activation [GO:0042113];
B cell cytokine production [GO:0002368];
B cell proliferation [GO:0042100];
chaperone-mediated protein complex assembly [GO:0051131];
isotype switching to IgG isotypes [GO:0048291];
MyD88-dependent toll-like receptor signaling pathway [GO:0002755];
negative regulation of apoptotic process [GO:0043066];
positive regulation of apoptotic process [GO:0043065];
positive regulation of interferon-alpha production [GO:0032727];
positive regulation of interferon-gamma production [GO:0032729];
positive regulation of interleukin-10 production [GO:0032733];
positive regulation of interleukin-12 production [GO:0032735];
positive regulation of interleukin-6 production [GO:0032755];
positive regulation of macrophage activation [GO:0043032];
positive regulation of T cell activation [GO:0050870];
positive regulation of T cell mediated immune response to tumor cell [GO:0002842];
protein maturation [GO:0051604];
protein refolding [GO:0042026];
protein stabilization [GO:0050821];
response to cold [GO:0009409];
response to unfolded protein [GO:0006986];
T cell activation [GO:0042110];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activity [GO:0016887];
ATP binding [GO:0005524];
chaperone binding [GO:0051087];
DNA replication origin binding [GO:0003688];
double-stranded RNA binding [GO:0003725];
lipopolysaccharide binding [GO:0001530];
p53 binding [GO:0002039];
poly(A RNA binding [GO:0044822];
single-stranded DNA binding [GO:0003697];
ubiquitin protein ligase binding [GO:0031625];
unfolded protein binding [GO:0051082]
Gene Ontology
(Cellular Component)
Complete annatation
cell surface [GO:0009986];
coated pit [GO:0005905];
coated vesicle [GO:0030135];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
early endosome [GO:0005769];
extracellular exosome [GO:0070062];
extracellular space [GO:0005615];
lipopolysaccharide receptor complex [GO:0046696];
membrane [GO:0016020];
mitochondrial inner membrane [GO:0005743];
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739];
protein complex [GO:0043234];
secretory granule [GO:0030141]
Protein-protein interaction109561
Phylogenetic treeP10809
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.851750710613544.44089209850063e-161.7430501486615e-14
AZA vs. DISU-0.1337351143809310.5965673434158340.955158276076142
AZA vs. IL70.5266127269297470.006143849174717110.247610263664468
AZA vs. SAHA0.07516030124055120.7597345714839770.935749414029693
DISU vs. CD3-2.997354901318531.55431223447522e-147.63182495846236e-13
DISU vs. IL70.651428995454070.009989729961518370.11737738049095
DISU vs. SAHA0.2093492113178130.4766899328032130.808571101591546
DMSO vs. AZA-0.08230063473218470.6220036013752371
DMSO vs. CD3-2.9454167435019400
DMSO vs. DISU0.04948124135261810.8391170530522290.982583769983649
DMSO vs. IL70.6162624245547710.0006134911811227490.0407661644166636
DMSO vs. SAHA0.1501292234163050.5276095822556930.828292084809954
HIV vs. Mock in Activation-0.1167435036343710.8509191110154080.999983755607037
HIV vs. Mock in Latency-0.1551914500144830.3459164881517740.999834320637052
IL7 vs. CD3-2.316465425876225.85653747720016e-121.51156831153832e-10
SAHA vs. CD3-2.80198679980691.55875312657372e-135.46288595755022e-12
SAHA vs. IL7-0.4550199230490770.09418403127072660.277833676590006
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.5009 0.02684

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.577785 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.989 0.982 1.014 0.955 0.836
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
0.47 <0.0001 -0.27 <0.0001 -0.17 0.0007 T-cell activation at 20 hpi
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4PJ1 X-ray 3.1Å A/B/C/D/E/F/G/H/I/J/K/L/M/N=27-556.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 11932435
Gag-Pol complexes with 23125841
Nef interacts with 23439083
Pol interacts with 23439083
Tat upregulates 23364796
HIV-1 virus replication enhanced by expression of human gene 23439083
Nef complexes with 23125841
Envelope transmembrane glycoprotein gp41 binds 10499815
Envelope surface glycoprotein gp120 complexes with 23125841
reverse transcriptase interacts with 23439083
retropepsin cleaves 22944692
Vpr downregulates 23874603
Pr55(Gag) complexes with 23125841
integrase stimulated by 11689615

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03018 RNA degradation - Homo sapiens (human)
hsa04940 Type I diabetes mellitus - Homo sapiens (human)
hsa05134 Legionellosis - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)