Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001379
UniProt IDP38646
Primary gene name(s)HSPA9
Synonym gene name(s)GRP75, HSPA9B, mt-HSP70
Protein nameStress-70 protein, mitochondrial
Protein functionChaperone protein which is implicated in the control of cell proliferation and cellular aging. Plays a role in the erythropoiesis process. {ECO:0000269|PubMed:21123823, ECO:0000269|PubMed:23501103}.
Subcellular locationMitochondrion {ECO:0000269|PubMed:22002106}. Nucleus, nucleolus {ECO:0000269|PubMed:22002106}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P38646
Gene Ontology
(Biological Process)
Complete annatation
erythrocyte differentiation [GO:0030218];
negative regulation of apoptotic process [GO:0043066];
negative regulation of erythrocyte differentiation [GO:0045647];
negative regulation of hematopoietic stem cell differentiation [GO:1902037];
protein export from nucleus [GO:0006611];
protein folding [GO:0006457]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
poly(A RNA binding [GO:0044822];
ubiquitin protein ligase binding [GO:0031625];
unfolded protein binding [GO:0051082]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
mitochondrial nucleoid [GO:0042645];
mitochondrion [GO:0005739];
myelin sheath [GO:0043209];
nucleolus [GO:0005730]
Protein-protein interaction109545
Phylogenetic treeP38646
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.681221114981635.45133789331409e-074.48730555587275e-06
AZA vs. DISU0.1356537566719920.5909728700702390.955158276076142
AZA vs. IL70.2979757144099120.1203667444751870.88372525059644
AZA vs. SAHA-0.1282230922010950.5985440332062330.867960823141006
DISU vs. CD3-1.558605522112452.56030803962304e-050.000170092919300475
DISU vs. IL70.1532951904850490.5420863532368450.850049733824155
DISU vs. SAHA-0.2623201881350840.3676711784760690.739865038264888
DMSO vs. AZA0.0003311574983698160.9984169403220391
DMSO vs. CD3-1.691986927719562.45440163415545e-071.97097871101022e-06
DMSO vs. DISU-0.1371823233494390.5731362710877350.930932477407238
DMSO vs. IL70.304940843901960.0891767825894280.555676159426118
DMSO vs. SAHA-0.1352945561451710.565384819679390.846518138453843
HIV vs. Mock in Activation-0.03981350610318820.9488897244019920.999983755607037
HIV vs. Mock in Latency-0.0585823576727210.7217089582315140.999834320637052
IL7 vs. CD3-1.375543225049882.5044730831425e-050.000173882647545863
SAHA vs. CD3-1.833922375700864.6119022323321e-074.06379343893987e-06
SAHA vs. IL7-0.429375687008930.07773779347804650.245973866265339
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.27678 0.0345308
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.023 0.996 0.962 0.913 0.826
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3N8E X-ray 2.8Å A/B=439-597.
4KBO X-ray 2.8Å A=52-431.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 11932435
Gag-Pol complexes with 23125841
Nef interacts with 21763498
Nef complexes with 23125841
Vpr interacts with 21763498
Envelope surface glycoprotein gp120 complexes with 23125841
Nef binds 22013042
Envelope surface glycoprotein gp120 inhibited by 12832005
Pr55(Gag) complexes with 23125841
Vpr competes with 10964507
Tat regulated by 10617616
Envelope surface glycoprotein gp120 upregulates 7906708
matrix stimulated by 10964507

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03018 RNA degradation - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)