Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001375
UniProt IDP34932
Primary gene name(s)HSPA4
Synonym gene name(s)APG2
Protein nameHeat shock 70 kDa protein 4
Protein functionunknown
Subcellular locationCytoplasm {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P34932
Gene Ontology
(Biological Process)
Complete annatation
chaperone-mediated protein complex assembly [GO:0051131];
protein import into mitochondrial outer membrane [GO:0045040];
response to unfolded protein [GO:0006986]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062]
Protein-protein interaction109540
Phylogenetic treeP34932
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.023652901002462.46903686385025e-093.27006358628743e-08
AZA vs. DISU0.06267530859876150.804104745922720.983438610789082
AZA vs. IL70.4021633939957590.03654410418023790.587890172301508
AZA vs. SAHA0.08654279677210.7233842601454470.923073348989435
DISU vs. CD3-1.973308691917341.42111494239039e-071.67358489387443e-06
DISU vs. IL70.3307610516512390.1890789303978030.563096804381579
DISU vs. SAHA0.02445690481316530.9332333507081530.98436042668566
DMSO vs. AZA-0.1457147920272830.3843902936213631
DMSO vs. CD3-2.180272676256936.02284888628901e-119.88390179432941e-10
DMSO vs. DISU-0.2101981467914420.388699869567260.86028854108019
DMSO vs. IL70.5551397791803680.002060891954894560.0885126672935485
DMSO vs. SAHA0.2246405762404180.3417546391273950.694055271026676
HIV vs. Mock in Activation-0.1085891980742930.8612339321190410.999983755607037
HIV vs. Mock in Latency-0.1577367800787970.3398639666591440.999834320637052
IL7 vs. CD3-1.612579750306029.20149945127235e-079.0649804724855e-06
SAHA vs. CD3-1.9627919559348.27515466150786e-088.88223509607716e-07
SAHA vs. IL7-0.319385783454530.1906086916853710.421010404308332
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -0.98004775
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.34962 0.0017721
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.01 0.963 1 0.987 0.862
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 11932435
1963958511932435
19639585
21738476
Vpr interacts with 21763498
Nef interacts with 21763498
Vif interacts with 21228271
23831493
24189052
Envelope surface glycoprotein gp120 inhibited by 12832005
Vpr competes with 10964507
19275587
20012529
Tat regulated by 10617616
Envelope surface glycoprotein gp120 upregulates 7906708
17676665
matrix stimulated by 10964507

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04530 Tight junction - Homo sapiens (human)
hsa04612 Antigen processing and presentation - Homo sapiens (human)
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