Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001373
UniProt IDP14625
Primary gene name(s)HSP90B1
Synonym gene name(s)GRP94, TRA1
Protein nameEndoplasmin
Protein functionMolecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors, By similarity. Functions in endoplasmic reticulum associated degradation, ERAD. Has ATPase activity. {ECO:0000250, ECO:0000269|PubMed:18264092}.
Subcellular locationEndoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P14625
Gene Ontology
(Biological Process)
Complete annatation
actin rod assembly [GO:0031247];
ATF6-mediated unfolded protein response [GO:0036500];
cellular response to ATP [GO:0071318];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
negative regulation of apoptotic process [GO:0043066];
protein folding in endoplasmic reticulum [GO:0034975];
protein transport [GO:0015031];
receptor-mediated endocytosis [GO:0006898];
regulation of phosphoprotein phosphatase activity [GO:0043666];
response to endoplasmic reticulum stress [GO:0034976];
response to hypoxia [GO:0001666];
retrograde protein transport, ER to cytosol [GO:0030970];
sequestering of calcium ion [GO:0051208];
toll-like receptor signaling pathway [GO:0002224]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calcium ion binding [GO:0005509];
low-density lipoprotein particle receptor binding [GO:0050750];
protein phosphatase binding [GO:0019903];
RNA binding [GO:0003723];
virion binding [GO:0046790]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endocytic vesicle lumen [GO:0071682];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum chaperone complex [GO:0034663];
endoplasmic reticulum lumen [GO:0005788];
endoplasmic reticulum membrane [GO:0005789];
extracellular exosome [GO:0070062];
extracellular matrix [GO:0031012];
extracellular region [GO:0005576];
focal adhesion [GO:0005925];
melanosome [GO:0042470];
membrane [GO:0016020];
midbody [GO:0030496];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886]
Protein-protein interaction113036
Phylogenetic treeP14625
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.03541239040802770.9138262764945250.943886351379213
AZA vs. DISU0.090068066762790.7255936599680830.974309534149196
AZA vs. IL70.009976701089477520.9584624396685920.999311006273513
AZA vs. SAHA-0.04393225339710370.8566344316276690.967119466023303
DISU vs. CD30.1137301114144380.7534408422444890.828400237490403
DISU vs. IL7-0.08910094284594180.7254152173752290.930882001147483
DISU vs. SAHA-0.1331249458050150.6488742226158380.89153300390882
DMSO vs. AZA-0.0630019544274070.7053777839908641
DMSO vs. CD3-0.03907932973971240.9027536992182720.933616014191204
DMSO vs. DISU-0.1549964640937990.5329820525245950.919875593426842
DMSO vs. IL70.08025698885755480.6540254344210920.927729425045836
DMSO vs. SAHA0.01212562867288290.9588518030491820.990327269994122
HIV vs. Mock in Activation-0.4822754302108880.4404167197249960.999983755607037
HIV vs. Mock in Latency-0.1591851658971470.3335090205385920.999834320637052
IL7 vs. CD30.05394206956976090.8668642317242720.915067201119122
SAHA vs. CD3-0.03325049563194040.9249936190716990.94775998364227
SAHA vs. IL7-0.05764902993633270.8123694125635110.918998613507119
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.578992 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.012 0.923 1.093 1.079 0.784
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00615 Rifabutin approved unknown other/unknown
DB02935 1-Methoxy-2-(2-Methoxyethoxy)Ethane experimental unknown unknown
DB03719 N-Ethyl-5&,39;-Carboxamido Adenosine experimental unknown unknown
DB02103 2-Chlorodideoxyadenosine experimental unknown unknown
DB03758 Radicicol experimental unknown unknown
DB08465 2-(3-AMINO-2,5,6-TRIMETHOXYPHENYL)ETHYL 5-CHLORO-2,4-DIHYDROXYBENZOATE experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4NH9 X-ray 2.7Å A=69-337.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
Nef requires 25496667
Nef cooperates with 24204260

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04657 IL-17 signaling pathway - Homo sapiens (human)
hsa04915 Estrogen signaling pathway - Homo sapiens (human)
hsa04918 Thyroid hormone synthesis - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05215 Prostate cancer - Homo sapiens (human)