Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001372
UniProt IDP08238
Primary gene name(s)HSP90AB1
Synonym gene name(s)HSP90B, HSPC2, HSPCB
Protein nameHeat shock protein HSP 90-beta
Protein functionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. {ECO:0000269|PubMed:16478993, ECO:0000269|PubMed:19696785}.
Subcellular locationCytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P08238
Gene Ontology
(Biological Process)
Complete annatation
cellular response to drug [GO:0035690];
cellular response to interleukin-4 [GO:0071353];
cellular response to organic cyclic compound [GO:0071407];
Fc-gamma receptor signaling pathway involved in phagocytosis [GO:0038096];
fibril organization [GO:0097435];
negative regulation of complement-dependent cytotoxicity [GO:1903660];
negative regulation of neuron apoptotic process [GO:0043524];
negative regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032435];
placenta development [GO:0001890];
positive regulation of cell differentiation [GO:0045597];
positive regulation of cell size [GO:0045793];
positive regulation of nitric oxide biosynthetic process [GO:0045429];
positive regulation of phosphoprotein phosphatase activity [GO:0032516];
positive regulation of protein binding [GO:0032092];
positive regulation of protein import into nucleus, translocation [GO:0033160];
positive regulation of protein localization to cell surface [GO:2000010];
positive regulation of protein serine/threonine kinase activity [GO:0071902];
positive regulation of transforming growth factor beta receptor signaling pathway [GO:0030511];
protein folding [GO:0006457];
protein stabilization [GO:0050821];
regulation of cellular response to heat [GO:1900034];
regulation of interferon-gamma-mediated signaling pathway [GO:0060334];
regulation of protein ubiquitination [GO:0031396];
regulation of type I interferon-mediated signaling pathway [GO:0060338];
response to cocaine [GO:0042220];
response to salt stress [GO:0009651];
response to unfolded protein [GO:0006986];
virion attachment to host cell [GO:0019062]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
CTP binding [GO:0002135];
dATP binding [GO:0032564];
double-stranded RNA binding [GO:0003725];
GTP binding [GO:0005525];
histone deacetylase binding [GO:0042826];
kinase binding [GO:0019900];
MHC class II protein complex binding [GO:0023026];
nitric-oxide synthase regulator activity [GO:0030235];
poly(A RNA binding [GO:0044822];
protein kinase regulator activity [GO:0019887];
TPR domain binding [GO:0030911];
UTP binding [GO:0002134]
Gene Ontology
(Cellular Component)
Complete annatation
apical plasma membrane [GO:0016324];
basolateral plasma membrane [GO:0016323];
brush border membrane [GO:0031526];
cell surface [GO:0009986];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
inclusion body [GO:0016234];
melanosome [GO:0042470];
membrane [GO:0016020];
mitochondrion [GO:0005739];
nucleoplasm [GO:0005654]
Protein-protein interaction109558
Phylogenetic treeP08238
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.709197834499987.54951656745106e-152.56275481316717e-13
AZA vs. DISU0.1589161645330110.5751234493255830.954304256505839
AZA vs. IL70.5478899758625240.004323628161943870.204294819075172
AZA vs. SAHA-0.1021615193034890.6744556940741740.9044342258228
DISU vs. CD3-2.562446368110622.252487085741e-116.41852369195837e-10
DISU vs. IL70.3797440181896310.1880215217007650.56214371887353
DISU vs. SAHA-0.2593000478339840.3737502598635940.743545424892329
DMSO vs. AZA0.01002890267887580.9522773712418641
DMSO vs. CD3-2.708781575029491.77635683940025e-155.76741719516482e-14
DMSO vs. DISU-0.1504000438625060.5868338952133990.934701463289767
DMSO vs. IL70.5450385445634160.002405003710157060.0956607676269596
DMSO vs. SAHA-0.1190461906554940.6126501999238440.872103223811296
HIV vs. Mock in Activation0.1715792583296170.7824127990780410.999983755607037
HIV vs. Mock in Latency-0.06531650100623190.6913388972353830.999834320637052
IL7 vs. CD3-2.154175736727531.22110543898657e-102.56347106989479e-09
SAHA vs. CD3-2.835255726873556.20614670765463e-142.39198544461267e-12
SAHA vs. IL7-0.652493011332740.007441127912427570.0509158248533514
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.687 0.008481

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.226935832
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.427027 0.00199466
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.992 1.036 1.013 0.909 0.908
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
214359_s_at 1.41 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02424 Geldanamycin experimental unknown unknown
DB02754 9-Butyl-8-(3,4,5-Trimethoxybenzyl)-9h-Purin-6-Amine experimental unknown unknown
DB05134 CNF1010 investigational unknown unknown
DB06070 SNX-5422 investigational unknown unknown
DB07594 4-[4-(2,3-DIHYDRO-1,4-BENZODIOXIN-6-YL)-3-METHYL-1H-PYRAZOL-5-YL]-6-ETHYLBENZENE-1,3-DIOL experimental unknown unknown
DB07877 8-(6-BROMO-BENZO[1,3]DIOXOL-5-YLSULFANYL)-9-(3-ISOPROPYLAMINO-PROPYL)-ADENINE experimental unknown unknown
DB08045 4-{4-[4-(3-AMINOPROPOXY)PHENYL]-1H-PYRAZOL-5-YL}-6-CHLOROBENZENE-1,3-DIOL experimental unknown unknown
DB08153 (5E)-14-CHLORO-15,17-DIHYDROXY-4,7,8,9,10,11-HEXAHYDRO-2-BENZOXACYCLOPENTADECINE-1,12(3H,13H)-DIONE experimental unknown unknown
DB08292 (5Z)-12-CHLORO-13,15-DIHYDROXY-4,7,8,9-TETRAHYDRO-2-BENZOXACYCLOTRIDECINE-1,10(3H,11H)-DIONE experimental unknown unknown
DB08293 (5E)-12-CHLORO-13,15-DIHYDROXY-4,7,8,9-TETRAHYDRO-2-BENZOXACYCLOTRIDECINE-1,10(3H,11H)-DIONE experimental unknown unknown
DB08346 (5Z)-13-CHLORO-14,16-DIHYDROXY-3,4,7,8,9,10-HEXAHYDRO-1H-2-BENZOXACYCLOTETRADECINE-1,11(12H)-DIONE experimental unknown unknown
DB03758 Radicicol experimental unknown unknown
DB08465 2-(3-AMINO-2,5,6-TRIMETHOXYPHENYL)ETHYL 5-CHLORO-2,4-DIHYDROXYBENZOATE experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1QZ2 X-ray 3.0Å G/H=720-724.
1UYM X-ray 2.4Å A=2-221.
2L6J NMR - B=720-724.
3FWV X-ray 2.2Å C/D=719-723.
3NMQ X-ray 2.2Å A=1-223.
3PRY X-ray 2.2Å A/B/C=284-543.
3UQ3 X-ray 2.6Å B/C=720-724.
5FWK EM 3.9Å A/B=1-724.
5FWL EM 9.0Å A/B=1-724.
5FWM EM 8.0Å A/B=1-724.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 21738476
retropepsin cleaves 22944692
capsid regulated by 23200770
Vpr upregulates 23874603
Tat upregulates 23166591
Gag-Pol incorporates 21738476
Tat downregulates 23287597

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04612 Antigen processing and presentation - Homo sapiens (human)
hsa04621 NOD-like receptor signaling pathway - Homo sapiens (human)
hsa04657 IL-17 signaling pathway - Homo sapiens (human)
hsa04659 Th17 cell differentiation - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa04915 Estrogen signaling pathway - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05215 Prostate cancer - Homo sapiens (human)