Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001366
UniProt IDP00492
Primary gene name(s)HPRT1
Synonym gene name(s)HPRT
Protein nameHypoxanthine-guanine phosphoribosyltransferase
Protein functionConverts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P00492
Gene Ontology
(Biological Process)
Complete annatation
adenine salvage [GO:0006168];
central nervous system neuron development [GO:0021954];
cerebral cortex neuron differentiation [GO:0021895];
cytolysis [GO:0019835];
dendrite morphogenesis [GO:0048813];
dopamine metabolic process [GO:0042417];
GMP catabolic process [GO:0046038];
GMP salvage [GO:0032263];
grooming behavior [GO:0007625];
guanine salvage [GO:0006178];
hypoxanthine metabolic process [GO:0046100];
hypoxanthine salvage [GO:0043103];
IMP metabolic process [GO:0046040];
IMP salvage [GO:0032264];
locomotory behavior [GO:0007626];
lymphocyte proliferation [GO:0046651];
positive regulation of dopamine metabolic process [GO:0045964];
protein homotetramerization [GO:0051289];
purine-containing compound salvage [GO:0043101];
purine nucleotide biosynthetic process [GO:0006164];
purine ribonucleoside salvage [GO:0006166];
response to amphetamine [GO:0001975];
striatum development [GO:0021756]
Gene Ontology
(Molecular Function)
Complete annatation
guanine phosphoribosyltransferase activity [GO:0052657];
hypoxanthine phosphoribosyltransferase activity [GO:0004422];
magnesium ion binding [GO:0000287];
nucleotide binding [GO:0000166];
protein homodimerization activity [GO:0042803]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062]
Protein-protein interaction109488
Phylogenetic treeP00492
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.721240174914543.18735279170035e-072.75141931709666e-06
AZA vs. DISU-0.4360026077220520.08678795617119770.614197958368373
AZA vs. IL70.3665736159572930.0585247562711980.69955880354938
AZA vs. SAHA-0.0337326833078450.8905730242674390.976874050805
DISU vs. CD3-2.169039725012519.88996096129569e-091.52434837965819e-07
DISU vs. IL70.7936122813474730.001819273200674990.0400414558375898
DISU vs. SAHA0.4029304078960380.1691625310962840.530803353352508
DMSO vs. AZA-0.1387533885518490.4130171704687681
DMSO vs. CD3-1.869152059151531.53769421640249e-081.58233356353166e-07
DMSO vs. DISU0.296162207835810.2274421364635320.739772327779506
DMSO vs. IL70.5120737199019750.00481099109178740.14040039803309
DMSO vs. SAHA0.09721509220397590.68172772267470.900623992674474
HIV vs. Mock in Activation-0.2253116254540760.7170959322090190.999983755607037
HIV vs. Mock in Latency-0.1055712798253590.5275915913129010.999834320637052
IL7 vs. CD3-1.346071955388983.76669594996937e-050.000247603175115082
SAHA vs. CD3-1.780185834103479.7987385383469e-077.94335609321612e-06
SAHA vs. IL7-0.4039241223149970.09877222786558470.28494442363008
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.320636 0.0160598
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.06 0.005

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.025 0.892 0.918 0.901 0.829
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01632 Alpha-Phosphoribosylpyrophosphoric Acid experimental unknown unknown
DB02309 5--Monophosphate-9-Beta-D-Ribofuranosyl Xanthine experimental unknown unknown
DB04356 9-Deazaguanine experimental unknown unknown
DB03153 3h-Pyrazolo[4,3-D]Pyrimidin-7-Ol experimental unknown unknown
DB00993 Azathioprine approved yes inhibitor
DB01033 Mercaptopurine approved yes inhibitor
DB00352 Tioguanine approved unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1BZY X-ray 2.0Å A/B/C/D=2-218.
1D6N X-ray 2.7Å A/B=5-218.
1HMP X-ray 2.5Å A/B=2-218.
1Z7G X-ray 1.9Å A/B/C/D=2-218.
2VFA X-ray 2.8Å A/B=49-160.
3GEP X-ray 2.6Å A/B=2-218.
3GGC X-ray 2.7Å A/B=2-218.
3GGJ X-ray 2.6Å A/B=2-218.
4IJQ X-ray 2.0Å A/B/C/D=2-218.
4KN6 X-ray 2.7Å A=3-218.
4RAB X-ray 2.2Å A/B/C/D=2-218.
4RAC X-ray 2.0Å A/B/C/D=2-218.
4RAD X-ray 2.0Å A/B/C/D/E/F/G/H=2-218.
4RAN X-ray 2.5Å A/B/C/D=2-218.
4RAO X-ray 1.8Å A/B/C/D=2-218.
4RAQ X-ray 2.5Å A/B/C/D=2-218.
5BRN X-ray 2.3Å A/B/C/D=1-218.
5BSK X-ray 2.6Å A/B/C/D=1-218.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr downregulates 23728617

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00983 Drug metabolism - other enzymes - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)